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Wild griffon vultures (Gyps fulvus) fed at supplementary feeding stations: Potential carriers of pig pathogens and pig-derived antimicrobial resistance?

Transboundary and Emerging Diseases publish this investigation article

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The carriage of two important pathogens of pigs, i.e. enterotoxigenic E. coli (ETEC) and Clostridioides difficile, was investigated in 104 cloacal samples from wild griffon vultures (Gyps fulvus) fed on pig carcasses at supplementary feeding stations (SFS), along with their level of antimicrobial resistance (AMR). E. coli was isolated from 90 (86.5%) samples but no ETEC was detected, likely because ETEC fimbriae confer the species specificity of the pathogen. Resistance to at least one antimicrobial agent was detected in 89.9% of E. coli isolates, being AMR levels extremely high (>70%) for tetracycline and streptomycin, and very high (>50%) for ampicillin and sulfamethoxazole-trimethoprim. Resistance to other critically important antimicrobials such as colistin and extended-spectrum cephalosporins was 2.2%, and 1.1%, respectively, and was encoded by the mcr-1 and blaSHV-12 genes. Multidrug resistance was displayed by 80% of the resistant E. coli and blaSHV-12 gene shared plasmid with other AMR genes. In general, resistance patterns in E. coli from vultures mirrored those found in pigs. C. difficile was detected in three samples (2.9%), two of them belonged to PCR-ribotype 078 and one to PCR-ribotype 126, both commonly found in pigs. All C. difficile isolates were characterized by a moderate to high level of resistance to fluoroquinolones and macrolides but susceptible to metronidazole or vancomycin, similar to what is usually found in C. difficile isolates from pigs. Thus, vultures may contribute somewhat to the environmental dissemination of some pig pathogens through their acquisition from pig carcasses and, more importantly, of AMR for antibiotics of critical importance for humans. However, the role of vultures would likely be much lesser than that of disposing pig carcasses at the SFS. The monitoring of AMR, and particularly of colistin resistant and ESLB-producing E. coli, should be considered in pig farms used as sources of carcasses for SFS




Sevilla E., Marin C., Delgado-Blas JF., Gonzalez-Zorn B., Vega S., Kuijper E., Bolea R. and Mainar-Jaime RC.




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Wild griffon vultures (Gyps fulvus) fed at supplementary feeding stations: Potential carriers of pig pathogens and pig-derived antimicrobial resistance?

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Wild griffon vultures (Gyps fulvus) fed at supplementary feeding stations: Potential carriers of pig pathogens and pig-derived antimicrobial resistance?


Participants:

Universidad de ZaragozaInstituto Universitario de Investigación Mixto Agroalimentario de Aragón (IA2). Universidad de Zaragoza (UNIZAR).

Universidad de ZaragozaDepartamento de Patología Animal. Facultad de Veterinaria. Universidad de Zaragoza (UNIZAR).

Universidad CEU Cardenal HerreraInstituto de Ciencias Biomédicas. Universidad CEU Cardenal Herrera (CEU-UCH).

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Leiden University Medical CentreDpt. of Medical Microbiology. Centre of Infectious Diseases. Leiden University Medical Centre.







Transboundary and Emerging Diseases
FACTOR YEAR Q
3.554 2018

NLMID: 101319538

PMID: 31901154

ISSN: 1865-1674



TITLE: Wild griffon vultures (Gyps fulvus) fed at supplementary feeding stations: Potential carriers of pig pathogens and pig-derived antimicrobial resistance?


JOURNAL: Transbound. Emerg. Dis.


PUBLISHER: Blackwell Verlag


AUTHORS: Sevilla E., Marin C., Delgado-Blas JF., Gonzalez-Zorn B., Vega S., Kuijper E., Bolea R. and Mainar-Jaime RC.


VISAVET PARTICIPANTS


3rd
José Francisco Delgado Blas
4th
Bruno González Zorn

DOI: https://doi.org/ 10.1111/tbed.13470


CITE THIS PUBLICATION:

Sevilla E., Marin C., Delgado-Blas JF., Gonzalez-Zorn B., Vega S., Kuijper E., Bolea R. and Mainar-Jaime RC. Wild griffon vultures (Gyps fulvus) fed at supplementary feeding stations: Potential carriers of pig pathogens and pig-derived antimicrobial resistance?. Transboundary and Emerging Diseases. In Press. (A). ISSN: 1865-1674. DOI: 10.1111/tbed.13470


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