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Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B

Investigación publicada en Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health

1 de enero de 1994

Three monoclonal antibodies (MAbs) which react specifically with staphylococcal enterotoxin B (SEB) were studied for their suitability for use in ELISA. One of them (MAb B14) worked well as a coating antibody; MAb B12 was shown to be a good probing antibody (conjugated with peroxidase) when ELISA plates were coated with MAb B14. This effective pair of MAbs (B14-B12PO) is able to detect 0.625 ng SEB ml, and to distinguish between SEB and other proteins present in food extracts




Goyache J., Orden JA., Blanco JL., Domenech A., Suarez G. y Gomez-Lucia E.




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Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B

Ver en NLM PubMed
Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B


Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







FACTOR YEAR Q
0.662 1997

NLMID: 100955260

PMID: 7597856

ISSN: 0931-1793



TÍTULO: Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B


REVISTA: J. Vet. Med. B Infect. Dis. Vet. Public Health


NUMERACIÓN: 41(10):639-44


AÑO: 1994


EDITORIAL: Blackwell Scientific Publications


AUTORES: Goyache J., Orden JA., Blanco JL., Domenech A., Suarez G. and Gomez-Lucia E.


PARTICIPANTES VISAVET


First
Joaquín Goyache Goñi
5th
Guillermo Suárez Fernández

DOI: https://doi.org/10.1111/j.1439-0450.1994.tb00274.x


CITA ESTA PUBLICACIÓN:

Goyache J., Orden JA., Blanco JL., Domenech A., Suarez G. y Gomez-Lucia E. Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health. 41(10):639-44. 1994. (A). ISSN: 0931-1793. DOI: 10.1111/j.1439-0450.1994.tb00274.x


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