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Evaluation of Volatile Organic Compounds Obtained from Breath and Feces to Detect Mycobacterium tuberculosis Complex in Wild Boar (Sus scrofa) in Doñana National Park, Spain

Investigación publicada en Pathogens

2 de mayo de 2020

The presence of Mycobacterium tuberculosis complex (MTBC) in wild swine, such as in wild boar (Sus scrofa) in Eurasia, is cause for serious concern. Development of accurate, efficient, and noninvasive methods to detect MTBC in wild swine would be highly beneficial to surveillance and disease management efforts in affected populations. Here, we describe the first report of identification of volatile organic compounds (VOC) obtained from the breath and feces of wild boar to distinguish between MTBC-positive and MTBC-negative boar. We analyzed breath and fecal VOC collected from 15 MTBC-positive and 18 MTBC-negative wild boar in Donaña National Park in Southeast Spain. Analyses were divided into three age classes, namely, adults (>2 years), sub-adults (12–24 months), and juveniles (<12 months). We identified significant compounds by applying the two-tailed statistical t-test for two samples assuming unequal variance, with an α value of 0.05. One statistically significant VOC was identified in breath samples from adult wild boar and 14 were identified in breath samples from juvenile wild boar. One statistically significant VOC was identified in fecal samples collected from sub-adult wild boar and three were identified in fecal samples from juvenile wild boar. In addition, discriminant function analysis (DFA) was used to build classification models for MTBC prediction in juvenile animals. Using DFA, we were able to distinguish between MTBC-positive juvenile wild boar and MTBC-negative juvenile wild boar using breath VOC or fecal VOC. Based on our results, further research is warranted and should be performed using larger sample sizes, as well as wild boar from various geographic locations, to verify these compounds as biomarkers for MTBC infection in this species. This new approach to detect MTBC infection in free-ranging wild boar potentially comprises a reliable and efficient screening tool for surveillance in animal populations




Nol P., Ionescu R., Welearegay TG., Barasona JA., Vicente J., Beleno-Saenz KJ., Barrenetxea I., Torres MJ., Ionescu F. y Rhyan J.




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Evaluation of Volatile Organic Compounds Obtained from Breath and Feces to Detect Mycobacterium tuberculosis Complex in Wild Boar (Sus scrofa) in Doñana National Park, Spain

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Evaluation of Volatile Organic Compounds Obtained from Breath and Feces to Detect Mycobacterium tuberculosis Complex in Wild Boar (Sus scrofa) in Doñana National Park, Spain


Participantes:

U.S. Department of AgricultureCenters for Epidemiology and Animal Health. Animal and Plant Health Inspection Services (APHIS). U.S. Department of Agriculture (USDA).

Universitat Rovira i VirgiliDepartment of Electronics. Electrical and Automatic Engineering. Universitat Rovira i Virgili (URV).

Uppsala UniversitetThe Ångström Laboratory, Division of Solid State Physics. Department of Materials Science and Engineering Sciences. Uppsala Universitet.

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Producción Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad Autónoma del CaribeFaculty of Engineering. Universidad Autónoma del Caribe.

Universidad ComplutenseDepartamento de Ingeniería Química. Facultad de Ciencias Químicas. Universidad Complutense (UCM).

Universidad de SevillaVirgen del Rocio Universitary Hospital. Universidad de Sevilla (US).

U.S. Department of AgricultureNational Veterinary Services Laboratories, Mycobacteria and Brucella Section. Animal and Plant Health Inspection Services (APHIS). U.S. Department of Agriculture (USDA).



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Pathogens
FACTOR YEAR Q
3.018 2019

NLMID: 101596317

PMID: 32370281

ISSN: 2076-0817



TÍTULO: Evaluation of Volatile Organic Compounds Obtained from Breath and Feces to Detect Mycobacterium tuberculosis Complex in Wild Boar (Sus scrofa) in Doñana National Park, Spain


REVISTA: Pathogens


NUMERACIÓN: 9(5):346


AÑO: 2020


EDITORIAL: MDPI


AUTORES: Nol P., Ionescu R., Welearegay TG., Barasona JA., Vicente J., Beleno-Saenz KJ., Barrenetxea I., Torres MJ., Ionescu F. and Rhyan J.


PARTICIPANTES VISAVET


José Ángel Barasona García-Arévalo

DOI: https://doi.org/10.3390/pathogens9050346


CITA ESTA PUBLICACIÓN:

Nol P., Ionescu R., Welearegay TG., Barasona JA., Vicente J., Beleno-Saenz KJ., Barrenetxea I., Torres MJ., Ionescu F. y Rhyan J. Evaluation of Volatile Organic Compounds Obtained from Breath and Feces to Detect Mycobacterium tuberculosis Complex in Wild Boar (Sus scrofa) in Doñana National Park, Spain. Pathogens. 9(5):346. 2020. (A). ISSN: 2076-0817. DOI: 10.3390/pathogens9050346


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