Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System
Sensors publica este artículo de investigación
15 de enero de 2021
More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis, in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected from free-ranging wild boar captured in Doñana National Park, Spain, with an electronic nose system based on organically-functionalized gold nanoparticles. The animals were separated by the age group for performing the analysis. Adult (>24 months) and sub-adult (12–24 months) animals were anesthetized before sample collection, whereas the juvenile (<12 months) animals were manually restrained while collecting the sample. Good accuracy was obtained for the adult and sub-adult classification models: 100% during the training phase and 88.9% during the testing phase for the adult animals, and 100% during both the training and testing phase for the sub-adult animals, respectively. The results obtained could be important for the further development of a non-invasive and less expensive detection method of bovine tuberculosis in wildlife populations
Beleno-Saenz KJ., Caceres-Tarazona JM., Nol P., Jaimes-Mogollon AL., Gualdron-Guerrero OE., Duran-Acevedo CM., Barasona JA., Vicente J., Torres MJ., Welearegay TG., Osterlund L., Rhyan J. y Ionescu F.
 | Faculty of Engineering. Universidad Autónoma del Caribe. |
 | GISM Group. Faculty of Engineering and Architecture. University of Pamplona. |
 | Veterinary Services. Animal and Plant Health Inspection. United States Department of Agriculture. |
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
 | Sanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM). |
 | Universidad de Sevilla (US). |
 | Biomedical Institute of Sevilla (IBiS). University Hospital Virgen del Rocío. |
 | The Ångström Laboratory, Division of Solid State Physics. Department of Materials Science and Engineering Sciences. Uppsala Universitet. |
 | Institute of Veterinary Medicine and Animal Sciences. Estonian University of Life Sciences (EMU). |
| Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
