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A new method for sampling African swine fever virus genome and its inactivation in environmental samples

Investigation published in Scientific reports

November 3rd, 2021

African swine fever (ASF) is currently the most dangerous disease for the global pig industry, causing huge economic losses, due to the lack of effective vaccine or treatment. Only the early detection of ASF virus (ASFV) and proper biosecurity measures are effective to reduce the viral expansion. One of the most widely recognized risks as regards the introduction ASFV into a country is infected animals and contaminated livestock vehicles. In order to improve ASF surveillance, we have assessed the capacity for the detection and inactivation of ASFV genome by using Dry-Sponges (3 M) pre-hydrated with a new surfactant liquid. We sampled different surfaces in ASFV-contaminated facilities, including animal skins, and the results were compared to those obtained using a traditional sampling method. The surfactant liquid successfully inactivated the virus, while ASFV DNA was well preserved for the detection. This is an effective method to systematically recover ASFV DNA from different surfaces and skin, which has a key applied relevance in surveillance of vehicles transporting live animals and greatly improves animal welfare. This method provides an important basis for the detection of ASFV genome that can be assessed without the biosafety requirements of a BSL-3 laboratory at least in ASF-affected countries, which may substantially speed up the early detection of the pathogen




Kosowska A., Barasona JA., Barroso-Arevalo S., Rivera B., Dominguez L. and Sanchez-Vizcaino JM..




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A new method for sampling African swine fever virus genome and its inactivation in environmental samples

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A new method for sampling African swine fever virus genome and its inactivation in environmental samples



Participants:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Scientific reports
FACTOR YEAR Q
4.379 2020

NLMID: 101563288

PMID: 34732758

ISSN: 2045-2322



TITLE: A new method for sampling African swine fever virus genome and its inactivation in environmental samples


JOURNAL: Sci Rep


NUMERACIÓN: 11(1):21560


AÑO: 2021


PUBLISHER: Nature Publishing Group


AUTHORS: Kosowska A., Barasona JA., Barroso-Arevalo S., Rivera B., Dominguez L. and Sanchez-Vizcaino JM..


VISAVET PARTICIPANTS


First
Aleksandra Kosowska
2nd
José Ángel Barasona García-Arévalo
3rd
Sandra Barroso Arévalo
4th
Belén Rivera Arroyo
5th
Lucas Domínguez Rodríguez
Last
José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/10.1038/s41598-021-00552-8


CITE THIS PUBLICATION:

Kosowska A., Barasona JA., Barroso-Arevalo S., Rivera B., Dominguez L. and Sanchez-Vizcaino JM. A new method for sampling African swine fever virus genome and its inactivation in environmental samples. Scientific reports. 11(1):21560. 2021. (A). ISSN: 2045-2322. DOI: 10.1038/s41598-021-00552-8


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