Application of the Enfer chemiluminescent multiplex ELISA system for the detection of Mycobacterium bovis infection in goats
Veterinary Microbiology publish this investigation article
January 27th, 2012
A study was conducted to optimise a multiplex serological immunoassay for use in identification of goats infected with Mycobacterium bovis. To assess assay specificity, 31 goats with a history of being free from M. bovis infection were used. To determine assay sensitivity, 180 Single Intradermal Comparative Tuberculin test (SICTT) positive goats were recruited. Additionally, 286 SICTT negative goats classed as potentially exposed animals present in the same positive herds were also included in the study. The results of the assay demonstrated a specificity of 100%. The multiplex assay detected 57/60 SICTT (95.0%) positive animals in one M. bovis infected herd and 120/120 (100%) in a second herd. In a separate experiment, 28 M. caprae culture confirmed infected goats from Spain were assayed, of which 24 (85.7%) were found positive in the test. The results show that inclusion of an antibody based assay can improve the ability to identify M. bovis and M. caprae infected goats. With further development and validation the multiplex assay may prove to be a useful tool for control of M. bovis and M. caprae infection in goats
Shuralev E., Quinn P., Doyle M., Duignan A., Kwok HF., Bezos J., Olwill SA., Gormley E., Aranaz A., Good M., Davis WC., Clarke J. and Whelan C.
 | Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Enfer Scientific. | |
 | UCD School of Agriculture, Food Science & Veterinary Medicine. University College Dublin (UCD). |
| Department of Agriculture, Fisheries and Food (DAFF). | |
| Fusion Antibodies Ltd. | |
| Department of Veterinary Microbiology and Pathology. College of Veterinary Medicine. Washington State University (WSU). | |
