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Simultaneous detection by multiplex PCR of rainbow trout pathogen microorganisms, in mediterranean area

New online article published in II Congreso Iberoamericano Virtual de Acuicultura

September 1st, 2003

Mata AI., Gibello A., Blanco MM., Dominguez L. and Fernandez-Garayzabal JF..


A multiplex PCR-assay (m-PCR) was designed for the simultaneous detection of the main pathogens involved in diseases for rainbow trout (Yersinia ruckeri, Streptococcus iniae and Lactococcus garvieae). Each of the three pairs of oligonucleotide primers used in the multiplex PCR amplified the targeted gene of the corresponding microorganism. However, the primers described for the PCR assay to detection of S. iniae were not specific enough, since amplifications with the DNA from Streptococcus agalactiae and Streptococcus difficilis were observed. Therefore, it could be necessary to find an useful alternative PCR method for the diagnosis of S. iniae infections





Related link: Simultaneous detection by multiplex PCR of rainbow trout pathogen microorganisms, in mediterranean area

Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). 111

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). 111








TITLE: PCR múltiple para la detección simultánea de microorganismos patógenos para la Trucha Arco Iris, en el área mediterránea


TYPE: Online article


AUTHORS: Mata AI., Gibello A., Blanco MM., Dominguez L. and Fernandez-Garayzabal JF..


VISAVET PARTICIPANTS


4th
Lucas Domínguez Rodríguez
Last
José Francisco Fernández-Garayzabal Fernández

JOURNAL: II Congreso Iberoamericano Virtual de Acuicultura


LANGUAJE: Spanish


NUMERATION: 461-467


DATE: September 1st, 2003


PUBLISHING COMPANY: CIVA



CITE THIS PUBLICATION:

Mata AI., Gibello A., Blanco MM., Dominguez L. and Fernandez-Garayzabal JF. PCR múltiple para la detección simultánea de microorganismos patógenos para la Trucha Arco Iris, en el área mediterránea.. II Congreso Iberoamericano Virtual de Acuicultura. 461-467. CIVA. 2003. (Online article)


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