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Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis

BioMed Research International (Journal of Biomedicine and Biotechnology) publish this investigation article

January 6th, 2014

Brucellosis is endemic in most parts of Egypt, where it is caused mainly by Brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant Brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 Brucella spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002-2007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as B. melitensis biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as B. abortus (n = 2) and B. suis biovar 1 (n = 2). MLVA-15 yielded a high discriminatory power (h = 0.801), indicating a high genetic diversity among the B. melitensis strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of B. suis from cattle in Egypt which, coupled with the finding of B. abortus, suggests a potential role of livestock as reservoirs of several zoonotic Brucella species in the region




Menshawy AMS., Perez-Sancho M., Garcia-Seco T., Hosein HI., Garcia N., Martinez I., Sayour AE., Goyache J., Azzam RAA., Dominguez L. and Alvarez J..




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Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis

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Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis



Participants:

Beni-Suef UniversityFaculty of Veterinary Medicine. Beni-Suef University.

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Animal Health Research InstituteDepartment of Brucellosis. Animal Health Research Institute (AHRI).

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







FACTOR YEAR Q
1.579 2014

NLMID: 101600173

PMID: 24511531

ISSN: 2314-6133



TITLE: Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis


JOURNAL: Biomed Res Int


NUMERACIÓN: 2014:353876


AÑO: 2014


PUBLISHER: Hindawi Pub. Co


AUTHORS: Menshawy AMS., Perez-Sancho M., Garcia-Seco T., Hosein HI., Garcia N., Martinez I., Sayour AE., Goyache J., Azzam RAA., Dominguez L. and Alvarez J..


VISAVET PARTICIPANTS


2nd
Marta Pérez Sancho
3rd
Teresa García-Seco Romero
5th
Nerea García Benzaquén
6th
Irene Martínez Alares
8th
Joaquín Goyache Goñi
10
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

DOI: https://doi.org/ 10.1155/2014/353876


CITE THIS PUBLICATION:

Menshawy AMS., Perez-Sancho M., Garcia-Seco T., Hosein HI., Garcia N., Martinez I., Sayour AE., Goyache J., Azzam RAA., Dominguez L. and Alvarez J. Assessment of genetic diversity of zoonotic Brucella spp. recovered from livestock in Egypt using multiple locus VNTR analysis. BioMed Research International (Journal of Biomedicine and Biotechnology). 2014:353876. 2014. (A). ISSN: 2314-6133. DOI: 10.1155/2014/353876


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