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Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain

Investigation article published in The Veterinary Record

October 1st, 2011

Two hundred and five isolates of Pasteurella multocida from pigs were phenotypically and genetically characterised by determining their biovar, capsular type, virulence-associated genes and pulsed-field gel electrophoresis (PFGE) profiles. All isolates were identified as P multocida subspecies multocida and most were assigned to biovar 3 (58 per cent) and biovar 2 (39.5 per cent). Biovar 1 represented 2.4 per cent of the isolates. According to the capsular type, the great majority of the isolates (79.0 per cent) belonged to capsular type A, 18.5 per cent belonged to capsular type D and 2.4 per cent were of capsular type F. All isolates harboured ompH, psl, oma87, ptfA, nanB, nanH, tonB, hgbA, sodA and sodC genes, while none of them possessed the transferrin-binding protein gene tbpA. The prevalence of toxA, pfhaA and hgbB genes was variable (7.8, 40.5 and 60.5 per cent of the isolates, respectively). After PFGE typing, isolates of biovar 2 and 3 were grouped in two different clusters (A and B) at a level of 45 per cent similarity. In addition, isolates of biovar 2 and 3 exhibited statistically significant differences (P<0.05) in the virulence-associated hgbB and pfhA genes (biovar 3 was hgbB(+) pfhA(-), while biovar 2 was hgbB- pfhA(+))




Garcia N., Fernandez-Garayzabal JF., Goyache J., Dominguez L. and Vela AI..




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Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain

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Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain


Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







The Veterinary Record
FACTOR YEAR Q
1.248 2011

NLMID: 31164

PMID: 21868435

ISSN: 0042-4900



TITLE: Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain


JOURNAL: Vet. Rec.


NUMERACIÓN: 169(14):362


AÑO: 2011


PUBLISHER: British Veterinary Medicine


AUTHORS: Garcia N., Fernandez-Garayzabal JF., Goyache J., Dominguez L. and Vela AI..


VISAVET PARTICIPANTS


First
Nerea García Benzaquén
2nd
José Francisco Fernández-Garayzabal Fernández
3rd
Joaquín Goyache Goñi
4th
Lucas Domínguez Rodríguez
Last
Ana Isabel Vela Alonso

DOI: https://doi.org/ 10.1136/vr.d4869


CITE THIS PUBLICATION:

Garcia N., Fernandez-Garayzabal JF., Goyache J., Dominguez L. and Vela AI. Associations between biovar and virulence factor genes in Pasteurella multocida isolates from pigs in Spain. The Veterinary Record. 169(14):362. 2011. (A). ISSN: 0042-4900. DOI: 10.1136/vr.d4869


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