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Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms

Investigation published in Veterinary Microbiology

March 1st, 2019

Antimicrobial resistant bacteria are rarely detected in laying hens and the objective of this longitudinal study was to test day-old chick as a source. Four different commercial batches raised on the same farm were monitored from day-old chick to laying hens using Escherichia coli as a model. Ten colonies from each of the eight samplings per batch were tested for antimicrobial susceptibility using 14 antimicrobials.
Overall (313 isolates), higher resistance percentages were detected for tetracycline (26.8%), followed by sulphonamides (16.3%), ampicillin (16.0 %) and quinolones (10.9% and 9.3% for ciprofloxacin and nalidixic acid, respectively). Resistance percentages of bacteria from day-old chicks were higher than those of pullets and hens (p < 0.05) for tetracycline, sulphonamides, trimethoprim and chloramphenicol.
Forty different phenotypic resistance profiles were detected, led by fully susceptible (182 isolates; 58.1%), and followed by single tetracycline (28 isolates; 8.9%) and ciprofloxacin/ nalidixic acid (11 isolates; 3.5%) profiles.
By whole-genome sequencing, 17 genes and mutations of five chromosomal genes related to resistance were detected, the most frequent being tetA, blaTEM-1B and sul1.
Using multilocus sequencing analysis, 58 different MLST types were detected, most of them only in a particular sample. The ST155 (27/142) was the most frequently detected, followed by ST10 (19/142) and ST48 (9/142).
The fate on the farm of the detected E. coli populations in old-day chicks was not clear, but our data suggest that they did not remain in the predominant faecal population of pullets and laying hens




Moreno MA., Garcia-Soto S., Hernandez M., Barcena C., Rodriguez-Lazaro D., Ugarte-Ruiz M. and Dominguez L..




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Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms

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Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms

Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Instituto Tecnológico Agrario de Castilla y LeónLaboratorio de Biología Molecular y Microbiología. Instituto Tecnológico Agrario de Castilla y León (ITACyL).

Universidad de BurgosUnidad de Microbiología. Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos (UBU).







Veterinary Microbiology
FACTOR YEAR Q
2.524 2017

NLMID: 7705469

PMID: 30827391

ISSN: 0378-1135



TITLE: Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 230:221-227


AÑO: 2019


PUBLISHER: Elsevier Science Publishers


AUTHORS: Moreno MA., Garcia-Soto S., Hernandez M., Barcena C., Rodriguez-Lazaro D., Ugarte-Ruiz M. and Dominguez L..


VISAVET PARTICIPANTS


First
Miguel Ángel Moreno Romo
4th
Carmen Bárcena Asensio
6th
María Ugarte Ruiz
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1016/j.vetmic.2019.02.007


CITE THIS PUBLICATION:

Moreno MA., Garcia-Soto S., Hernandez M., Barcena C., Rodriguez-Lazaro D., Ugarte-Ruiz M. and Dominguez L. Day-old chicks are a source of antimicrobial resistant bacteria for laying hen farms. Veterinary Microbiology. 230:221-227. 2019. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2019.02.007


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