Detection and characterization of clinical and fecal Escherichia coli isolates in laying hens
PhD Thesis defense by Magdalena Gibert Perelló at the Faculty of Veterinary Medicine of the Complutense University of Madrid
July 10th, 2009
The main objective of this study was the characterization of clinical (causing colibacillosis in lying hens) and faecal E. coli isolates. After performing the isolation and biochemical identification of the strains, analysis of serogroup, antimicrobial sensitivity, patotype (detection of virulence factors by PCR) and pulsotype (using Pulsed Field-Gel Electrophoresis, PFGE) was carried out. Furthermore, presence or absence of virulence factors and molecular characterization were analyzed by a comparative study with faecal isolates
