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Determination of patulin by diphasic dialysis extraction and thin-layer chromatography

Investigation published in Journal of Food Protection

January 1st, 1992

A quick, simple, and economical semiquantitative method has been devised for the detection of patulin in apple juice. The mycotoxin is extracted from the sample using ethyl acetate in a diphasic dialysis system. No purification or cleanup stages are required. Patulin is detected by thin-layer chromatography using a 3-methyl-2-benzothiazolinone-hydrazone solution as spray reagent. The limit of detection is 50 micrograms/L and recovery is 65% at 100 micrograms/L.




Prieta J., Moreno MA., Blanco JL., Suarez G. and Dominguez L..








Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







FACTOR YEAR Q
1.687 2005

NLMID: 7703944

ISSN: 0362-028X



TITLE: Determination of patulin by diphasic dialysis extraction and thin-layer chromatography


JOURNAL: J Food Prot


NUMERACIÓN: 55:1001-1002


AÑO: 1992


PUBLISHER: International Association for Food Protection


AUTHORS: Prieta J., Moreno MA., Blanco JL., Suarez G. and Dominguez L..


3rd
José Luis Blanco Cancelo
Last
Lucas Domínguez Rodríguez


CITE THIS PUBLICATION:

Prieta J., Moreno MA., Blanco JL., Suarez G. and Dominguez L. Determination of patulin by diphasic dialysis extraction and thin-layer chromatography. Journal of Food Protection. 55:1001-1002. 1992. (A). ISSN: 0362-028X.


UNITS: