Determination of patulin by reversed-phase high-performance liquid chromatography with extraction by diphasic dialysis
Investigation published in The Analyst
March 1st, 1993
A simple and economical method has been developed for the determination of patulin in apple juice. The sample is extracted with ethyl acetate in a diphasic dialysis system, and the extract is cleaned up by elution from a Sep-Pak cartridge. Patulin is detected and determined by reversed-phase high-performance liquid chromatography using a Novapak C18 column and an ultraviolet detector. The lower detection limit is 1 microgram l-1 and the recovery is 85% at the 20 micrograms l-1 level
Prieta J., Moreno MA., Bayo FJ., Diaz S., Suarez G., Dominguez L., Canela R. and Sanchis V.
Participants:
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Servicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
