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Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples

Research in Veterinary Science publish this investigation article

October 1st, 2013

DNA-based methods have emerged as an additional tool for Brucella infection-confirmation at a herd level. However, their implementation may require the use of specialized equipment. In this context the recently developed loop-mediated isothermal amplification (LAMP) technique may constitute an additional and cost-effective tool for rapid and specific DNA detection, especially in low income areas. In the present study the usefulness of a newly developed LAMP assay aiming at the multicopy-IS711 sequence was assessed on a variety of clinical samples (n=81 from abortions and ewes; cattle, n=3; swine, n=4) that were analyzed in parallel using real-time PCR and bacteriology. Although overall sensitivities obtained with the three methods were comparable (p>0.05), our results highlighted the complementarity between bacteriology and molecular-based methods for increased sensitivity. Significant differences (p<0.05) were observed with all techniques depending on the nature of the sample. Our results demonstrate the potential of the IS711-LAMP technique for direct Brucella detection




Perez-Sancho M., Garcia-Seco T., Arrogante L., Garcia N., Martinez I., Diez-Guerrier A., Perales A., Goyache J., Dominguez L. and Alvarez J..




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Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples

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Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples


Participants:

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Ministerio de Agricultura, Alimentación y Medio AmbienteLaboratorio Central de Sanidad Animal (LCSA). Ministerio de Agricultura, Alimentación y Medio Ambiente (MAGRAMA).

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







Research in Veterinary Science
FACTOR YEAR Q
1.511 2013

NLMID: 401300

PMID: 23714043

ISSN: 0034-5288



TITLE: Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples


JOURNAL: Res. Vet. Sci.


NUMERACIÓN: 95(2):489-94


AÑO: 2013


PUBLISHER: Elsevier


AUTHORS: Perez-Sancho M., Garcia-Seco T., Arrogante L., Garcia N., Martinez I., Diez-Guerrier A., Perales A., Goyache J., Dominguez L. and Alvarez J..


VISAVET PARTICIPANTS


First
Marta Pérez Sancho
2nd
Teresa García-Seco Romero
4th
Nerea García Benzaquén
5th
Irene Martínez Alares
6th
Alberto Antoine Díez Guerrier
8th
Joaquín Goyache Goñi
9th
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

DOI: https://doi.org/ 10.1016/j.rvsc.2013.05.002


CITE THIS PUBLICATION:

Perez-Sancho M., Garcia-Seco T., Arrogante L., Garcia N., Martinez I., Diez-Guerrier A., Perales A., Goyache J., Dominguez L. and Alvarez J. Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples. Research in Veterinary Science. 95(2):489-94. 2013. (A). ISSN: 0034-5288. DOI: 10.1016/j.rvsc.2013.05.002


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