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Discovery of Stable and Variable Differences in the Mycobacterium avium subsp. paratuberculosis Type I, II, and III Genomes by Pan-Genome Microarray Analysis

Applied and Environmental Microbiology publish this investigation article

February 1st, 2009

Mycobacterium avium subsp. paratuberculosis is an important animal pathogen widely disseminated in the environment that has also been associated with Crohn`s disease in humans. Three M. avium subsp. paratuberculosis genomotypes are recognized, but genomic differences have not been fully described. To further investigate these potential differences, a 60-mer oligonucleotide microarray (designated the MAPAC array), based on the combined genomes of M. avium subsp. paratuberculosis (strain K-10) and Mycobacterium avium subsp. hominissuis (strain 104), was designed and validated. By use of a test panel of defined M. avium subsp. paratuberculosis strains, the MAPAC array was able to identify a set of large sequence polymorphisms (LSPs) diagnostic for each of the three major M. avium subsp. paratuberculosis types. M. avium subsp. paratuberculosis type II strains contained a smaller genomic complement than M. avium subsp. paratuberculosis type I and M. avium subsp. paratuberculosis type III genomotypes, which included a set of genomic regions also found in M. avium subsp. hominissuis 104. Specific PCRs for genes within LSPs that differentiated M. avium subsp. paratuberculosis types were devised and shown to accurately screen a panel (n = 78) of M. avium subsp. paratuberculosis strains. Analysis of insertion/deletion region INDEL12 showed deletion events causing a reduction in the complement of mycobacterial cell entry genes in M. avium subsp. paratuberculosis type II strains and significantly altering the coding of a major immunologic protein (MPT64) associated with persistence and granuloma formation. Analysis of MAPAC data also identified signal variations in several genomic regions, termed variable genomic islands (vGIs), suggestive of transient duplication/deletion events. vGIs contained significantly low GC% and were immediately flanked by insertion sequences, integrases, or short inverted repeat sequences. Quantitative PCR demonstrated that variation in vGI signals could be associated with colony growth rate and morphology




Castellanos E., Aranaz A., Gould KA., Linedale R., Stevenson K., Alvarez J., Dominguez L., de Juan L., Hinds J. and Bull TJ.




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Discovery of Stable and Variable Differences in the Mycobacterium avium subsp. paratuberculosis Type I, II, and III Genomes by Pan-Genome Microarray Analysis

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Discovery of Stable and Variable Differences in the Mycobacterium avium subsp. paratuberculosis Type I, II, and III Genomes by Pan-Genome Microarray Analysis



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Division of Cellular and Molecular Medicine. St George´s University of London (SGUL).

Division of Control of Bacterial Diseases. Moredun Research Institute.







Applied and Environmental Microbiology
FACTOR YEAR Q
3.686 2009

NLMID: 7605801

PMID: 19047395

ISSN: 0099-2240



TITLE: Discovery of Stable and Variable Differences in the Mycobacterium avium subsp. paratuberculosis Type I, II, and III Genomes by Pan-Genome Microarray Analysis


JOURNAL: Appl. Environ. Microbiol.


NUMERACIÓN: 75(3):676-86


AÑO: 2009


PUBLISHER: American Society for Microbiology


AUTHORS: Castellanos E., Aranaz A., Gould KA., Linedale R., Stevenson K., Alvarez J., Dominguez L., de Juan L., Hinds J. and Bull TJ.


VISAVET PARTICIPANTS


6th
Julio Álvarez Sánchez
7th
Lucas Domínguez Rodríguez
8th
Lucía de Juan Ferré

DOI: https://doi.org/10.1128/AEM.01683-08


CITE THIS PUBLICATION:

Castellanos E., Aranaz A., Gould KA., Linedale R., Stevenson K., Alvarez J., Dominguez L., de Juan L., Hinds J. and Bull TJ. Discovery of Stable and Variable Differences in the Mycobacterium avium subsp. paratuberculosis Type I, II, and III Genomes by Pan-Genome Microarray Analysis. Applied and Environmental Microbiology. 75(3):676-86. 2009. (A). ISSN: 0099-2240. DOI: 10.1128/AEM.01683-08


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