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Environmental DNA: A Promising Factor for Tuberculosis Risk Assessment in Multi-Host Settings

PLoS ONE publish this investigation article

May 29th, 2020

Attaining and maintaining the Official Tuberculosis Free status continues to be a challenge when several domestic and wild hosts contribute to the maintenance of the Mycobacterium tuberculosis complex (MTC). Local tuberculosis hotspots are sometimes identified in cattle in low-prevalence regions. We have, therefore, studied one such hotspot in depth in order to produce an epidemiological diagnosis. Host population size and MTC prevalence were estimated in selected wildlife and in livestock, while on-cattle environmental DNA detection was additionally used as a proxy for risk of exposure at the farm (herd) level. Positive skin test reactors were found on16 of the 24 cattle farms studied in the period 2012-2016. Although all goats tested negative to the skin test during this period, MTC was confirmed in four sheep at slaughter, thus indicating an unknown prevalence of infection in this host species. With regard to wildlife, the prevalence of MTC infection based on culture was 8.8% in the case of wild boar (Sus scrofa), and the only road-killed badger (Meles meles) submitted for culture tested positive. Two criteria were employed to divide the cattle farms into higher or lower risk: tuberculosis testing results and environmental DNA detection. Environmental MTC DNA detection yielded significant differences regarding "use of regional pastures" and "proximity to woodland". This study suggests that on-animal environmental DNA sampling may help when assessing contact risk as regards MTC in livestock at the herd level. This tool opens up new avenues of epidemiological research in complex multi-host settings




Martinez-Guijosa J., Romero B., Infantes-Lorenzo JA., Diez E., Boadella M., Balseiro A., Veiga M., Navarro D., Moreno I., Ferreres J., Dominguez M., Fernandez C., Dominguez L. and Gortazar C.




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Environmental DNA: A Promising Factor for Tuberculosis Risk Assessment in Multi-Host Settings

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Environmental DNA: A Promising Factor for Tuberculosis Risk Assessment in Multi-Host Settings


Participants:

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno de NavarraServicio de Ganadería. Departamento de Desarrollo Rural. Medio ambiente y Administración Local. Gobierno de Navarra.

SabiotecSabiotec.

Gobierno del Principado de AsturiasServicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA). Gobierno del Principado de Asturias.

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).







PLoS ONE
FACTOR YEAR Q
2.740 2019

NLMID: 101285081

PMID: 32470035

ISSN: 1932-6203



TITLE: Environmental DNA: A Promising Factor for Tuberculosis Risk Assessment in Multi-Host Settings


JOURNAL: PLoS ONE


NUMERACIÓN: 15(5):e0233837


AÑO: 2020


PUBLISHER: Public Library Science


AUTHORS: Martinez-Guijosa J., Romero B., Infantes-Lorenzo JA., Diez E., Boadella M., Balseiro A., Veiga M., Navarro D., Moreno I., Ferreres J., Dominguez M., Fernandez C., Dominguez L. and Gortazar C.


VISAVET PARTICIPANTS


2nd
Beatriz Romero Martínez
13th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1371/journal.pone.0233837


CITE THIS PUBLICATION:

Martinez-Guijosa J., Romero B., Infantes-Lorenzo JA., Diez E., Boadella M., Balseiro A., Veiga M., Navarro D., Moreno I., Ferreres J., Dominguez M., Fernandez C., Dominguez L. and Gortazar C. Environmental DNA: A Promising Factor for Tuberculosis Risk Assessment in Multi-Host Settings. PLoS ONE. 15(5):e0233837. 2020. (A). ISSN: 1932-6203. DOI: 10.1371/journal.pone.0233837


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