Poster communication in One Health EJP Annual Scientific Meeting

April 11^st, 2022

Lopez-Chavarrias V., Wieczorek K., Osek J., Dieguez B., Torre-Fuentes L., Ugarte-Ruiz M., Moreno MA., Dominguez L. and Alvarez J.

Campylobacteriosis caused by Campylobacter jejuni remains the most important food-borne zoonosis in the EU, mostly associated to poultry products (1). Here, we compare C. jejuni isolates from humans and poultry recovered from Poland and Spain, to determine to what extent these two distant countries share antimicrobial resistance markers (RMs) and MLST types, as suggested by previous research (2).
Methods
In total, 83 Polish (15 humans, 68 broilers) and 222 Spanish isolates (146 humans, 62 broilers previously sequenced, 14 broilers newly sequenced) from 2010 to 2018 were included in the study. Human strains were recovered from faeces of hospital sporadic cases and broiler isolates were collected at abattoirs. Whole genome sequencing (Illumina) was used to identify the main RMs and MLST profiles, and the latter were analysed for clustering using the Minimum Spanning Tree (MST) algorithm (Bionumerics).
Results
The Polish MST showed two well defined clonal complexes (CCs) (ST-6411, ST-353), of
mainly broiler strains with little ST variation, whereas five main CCs (ST-443, ST-353, ST-21, ST-45, ST-828), of broiler and human strains with more distant STs were found in the Spanish MST. Polish isolates from both sources and Spanish broiler isolates harboured rep34 gene plasmid replicons. Plasmid encoded resistance gene cat(pC194) was identified in a Polish broiler isolate

	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Department of Hygiene of Food of Animal Origin. National Veterinary Research Institute (PIWET).
	Laboratorio Central de Veterinaria (LCV). Ministerio de Agricultura, Pesca y Alimentación (MAPA).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).