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Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats

Veterinary sciences publish this investigation article

February 28th, 2024

Paratuberculosis (PTB) and tuberculosis (TB) are two mycobacterial diseases with a severe economic and health impact on domestic ruminants. The ante mortem diagnosis of PTB is hampered, among other factors, by the limited sensitivity of all the available diagnostic techniques. Since TB-infected goats subjected to the comparative intradermal tuberculin test (CITT) may experience a booster effect on their antibody titer and a potential enhancement to the sensitivity of humoral techniques for tuberculosis, in the present study we aimed to evaluate this diagnostic strategy on the humoral diagnosis of PTB in serum and milk samples collected from a caprine herd that was TB free and PTB infected. The results from 120 goats indicated a significant increase (p < 0.001) in the quantitative response detected using an ELISA technique, conducted using serum and milk samples taken 15 and 30 days after performing a CITT (day 0 of the study); although, it did not translate into a significant increase in the number of reactors during any of the testing events (0, 3,15, 30 and 60 days post-CITT). Additionally, the number of ELISA-positive animals was higher for the serum versus the milk samples at both 15 and 30 days post-CITT. The increase in the quantitative ELISA result suggested a diagnostic strategy that maximizes ELISA sensitivity, mainly using serum samples, in PTB-infected herds; although, it may depend on individual differences and the interpretation criteria




Velasco-Reinaldos C., Ortega J., Gómez-Buendía A., Grau A., Lopez M., Alvarez J., Romero B., de Juan L. and Bezos J..




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Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats

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Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats



Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Junta de Castilla y LeónConsejería de Agricultura y Ganadería. Junta de Castilla y León.

Junta de Castilla y LeónServicio Territorial de Agricultura y Ganadería. Delegación Territorial de Ávila. Junta de Castilla y León.

Universidad ComplutenseServicio de Ensayos Clínicos (SEC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Veterinary sciences
FACTOR YEAR Q
2.000 2023

NLMID: 1015254153

PMID: 38535839

ISSN: 2306-7381



TITLE: Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats


JOURNAL: Vet Sci


NUMERACIÓN: 11(3):105


AÑO: 2024


PUBLISHER: MDPI


AUTHORS: Velasco-Reinaldos C., Ortega J., Gómez-Buendía A., Grau A., Lopez M., Alvarez J., Romero B., de Juan L. and Bezos J..


First
Carlos Velasco Reinaldos
3rd
Alberto Gómez Buendía
6th
Julio Álvarez Sánchez
7th
Beatriz Romero Martínez
8th
Lucía de Juan Ferré
Last
Javier Bezos Garrido

DOI: https://doi.org/10.3390/vetsci11030105


CITE THIS PUBLICATION:

Velasco-Reinaldos C., Ortega J., Gómez-Buendía A., Grau A., Lopez M., Alvarez J., Romero B., de Juan L. and Bezos J. Evaluation of the Effect of a Recent Comparative Intradermal Tuberculin Test on the Humoral Diagnosis of Paratuberculosis Using Serum and Milk Samples from Goats. Veterinary sciences. 11(3):105. 2024. (A). ISSN: 2306-7381. DOI: 10.3390/vetsci11030105


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