Home \ Outreach \


Evaluation of the effect of colistin reduction in pigs on Mcr-1 detection

Conference in One Health EJP Annual Scientific Meeting

June 9th, 2021

Miguela-Villoldo P., Moreno MA., Aguero M., Saez JL., Dominguez L. and Ugarte-Ruiz M.

Aim: Since the first description of the plasmid-mediated mcr-1 gene in Enterobacteraceae, the European Medicines Agency (EMA) and the Spanish Ministry of Agriculture (MAPA), recommended limiting the use of polymixins in animals, especially
in food-producing animals. Consequently, the Spanish Medicines and Healthcare Products Agency created a strategic plan to reduce the consumption of colistin in the pig industry. This study aims to analyze the evolution and current status of the
prevalence of mcr-1 gene in Spanish food-producing pigs population.
Methods: Samples from the caecal contents of healthy pigs (N=244) were sampled at slaughterhouses in different Spanish regions from January to December 2019. Direct DNA extraction from pig caecal samples was carried out coupled by a real-time
SYBR® Green I PCR assay for quantitative detection of the mcr-1 gene. Statistical analysis was performed using T-Test. Data was previously normalised by logarithmic transformation.
Results: From 244 samples, mcr-1 gene was quantified in 67 (27.5%), with a median of 2.69 Log10 copies/mg faeces. Comparing these data with our previous study (Miguela-Villoldo et al, 2019), where we analysed the prevalence of colistin resistance in pigs
from 2012 to 2017, we noticed a significantly decrease of the mcr-1 prevalence in pigs from the 70% reached in 2015.
Conclusions: The levels of mcr-1 show a declining trend that seems to correlate with variations in colistin sales in Spain, reaching similar values to those obtained in 2012 and 2013. So, the EMA recommendations and the Spanish program are playing an effective role in the fight against colistin resistance in pig production




Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Ministerio de Agricultura, Pesca y AlimentaciónLaboratorio Central de Veterinaria (LCV). Ministerio de Agricultura, Pesca y Alimentación (MAPA).

Ministerio de Agricultura, Pesca y AlimentaciónSubdirección General de Sanidad e Higiene Animal y Trazabilidad. Dirección General de la Producción Agraria. Ministerio de Agricultura, Pesca y Alimentación (MAPA).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).


Link to One Health EJP Annual Scientific Meeting





publiDB

One Health EJP Annual Scientific Meeting


One Health EJP Annual Scientific Meeting
Jun 9th-11st, 2021
Copenhagen
Denmark

TITLE: Evaluation of the effect of colistin reduction in pigs on Mcr-1 detection


TYPE: Oral communication


AUTHORS: Miguela-Villoldo P., Moreno MA., Aguero M., Saez JL., Dominguez L. and Ugarte-Ruiz M.


VISAVET PARTICIPANTS


First
Pedro Miguela Villoldo
2nd
Miguel Ángel Moreno Romo
5th
Lucas Domínguez Rodríguez
Last
María Ugarte Ruiz

DATE: June 9th, 2021


CITE THIS COMMUNICATION:

Miguela-Villoldo P., Moreno MA., Aguero M., Saez JL., Dominguez L. and Ugarte-Ruiz M. Evaluation of the effect of colistin reduction in pigs on Mcr-1 detection. One Health EJP Annual Scientific Meeting, National Food Institute. Technical University of Denmark, Statens Serum Institut, Copenhagen, Denmark, June 9th, 2021. (Oral communication)


UNITS: