Flavobacterium tructae sp. nov. and Flavobacterium piscis sp. nov. isolated from farmed rainbow trout (Oncorhynchus mykiss)
Investigation article published in International Journal of Systematic and Evolutionary Microbiology
February 1st, 2014
Four Gram-staining-negative, catalase- and oxidase-positive, pale-orange pigmented bacterial strains (435-08T, 47B-3-09, 412R-09T and 60B-3-09) were isolated from diseased rainbow trout. Analysis of their 16S rRNA gene sequences showed their adscription to the genus Flavobacterium. Strains formed two phylogenetic groups represented by strains 435-08T and 47B-3-09 (group A), and strains 412R-09T and 60B-3-09 (group B) displaying 16S rRNA sequence similarities greater than 99.8-99.9% within their respective groups. Strain 435-08T exhibited the highest levels of similarity with Flavobacterium aquidurense WB-1.1.56T (98.6% sequence similarity) and strain 412R-09T with Flavobacterium frigidimaris KUC-1T and Flavobacterium aquidurense WB-1.1.56T (98.9% and 98.6% sequence similarity, respectively). DNA-DNA hybridization studies showed low levels of relatedness between strain 435-08T and strain 412R-09T and between both strains and their most closely related Flavobacterium species. The genomic DNA G+C contents of strains 435-08T and 412R-09T were 36.2 and 34.3 mol%, respectively. The predominant respiratory quinone of both strains was MK-6 and the major fatty acids were iso-C15:0, C16:1 ω7c and C15:0. Both groups of strains were distinguished between them and from related Flavobacterium species by a number of phenotypic properties. Phylogenetic, genotypic and phenotypic evidences indicate that strains of group A and B represent two new species of the genus Flavobacterium, for which the names Flavobacterium tructae sp. nov (type strain 435-08T =CECT 7791T = CCUG 60100T) and Flavobacterium piscis sp. nov. (type strain 412R-09T =CECT 7911T = CCUG 60099T), are proposed
Zamora L., Vela AI., Sanchez-Porro C., Palacios MA., Moore ERB., Dominguez L., Ventosa A. and Fernandez-Garayzabal JF..
 | Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
| Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM). |
| Piscifactoría Zorita Illana, S.A. (PISZOLLA). | |
 | Facultad de Farmacia. Universidad de Sevilla (US). |
| Sahlgrenska Academy. University of Gothenburg. | |
