Home \ Research \ Scientific publications \


Fluoroquinolone efflux in Streptococcus suis is mediated by SatAB and not by SmrA

Antimicrobial Agents and Chemotherapy publish this investigation article

December 1st, 2011

Streptococcus suis is an emerging zoonotic pathogen. However, with the lack of an effective vaccine, antibiotics remain the main tool to fight against infections caused by this pathogen. We have previously observed a reserpine-sensitive fluoroquinolone-efflux phenotype in this species. Here, SatAB and SmrA, two pumps belonging to the ATP Binding Cassette and the Major Facilitator Superfamily respectively, have been analyzed in the fluoroquinolone-resistant clinical isolate BB1013. Genes encoding these pumps were overexpressed either constitutively or in the presence of ciprofloxacin in this strain. These genes could not be cloned onto plasmids in E. coli despite strong expression repression. Finally, site directed insertion of smrA and satAB in the amy locus of Bacillus subtilis chromosome using ligated PCR amplicons allowed for the functional expression and study of both pumps. Results showed that SatAB is a narrow spectrum fluoroquinolone exporter (norfloxacin and ciprofloxacin), susceptible to reserpine, whereas SmrA was not involved in fluoroquinolone extrusion. Chromosomal integration in Bacillus is a novel method for studying efflux pumps from Gram-positive bacteria, which enabled us to demonstrate the implication of SatAB, and not of SmrA, in fluoroquinolone efflux in S. suis




Escudero JA., San Millan A., Gutierrez B., Hidalgo L., La Ragione RM., Abuoun M., Galimand M., Ferrandiz MJ., Dominguez L., de la Campa AG. and Gonzalez-Zorn B..




See this article
Fluoroquinolone efflux in Streptococcus suis is mediated by SatAB and not by SmrA

See it on NLM PubMed
Fluoroquinolone efflux in Streptococcus suis is mediated by SatAB and not by SmrA


Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Department for Environment, Food and Rural AffairsAnimal Health and Veterinary Laboratories Agency (AHVLA). Department for Environment, Food and Rural Affairs (Defra).

University of SurreyFaculty of Health and Medical Sciences. University of Surrey.

Institut PasteurInstitut Pasteur.

Instituto de Salud Carlos IIICentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES). Instituto de Salud Carlos III (ISCIII).

Instituto de Salud Carlos IIIServicio de Bacteriología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Consejo Superior de Investigaciones CientíficasConsejo Superior de Investigaciones Científicas (CSIC).







Antimicrobial Agents and Chemotherapy
FACTOR YEAR Q
4.841 2011

NLMID: 315061

PMID: 21930876

ISSN: 0066-4804



TITLE: Fluoroquinolone efflux in Streptococcus suis is mediated by SatAB and not by SmrA


JOURNAL: Antimicrob. Agents Chemother.


NUMERACIÓN: 55(12):5850-60


AÑO: 2011


PUBLISHER: American Society for Microbiology


AUTHORS: Escudero JA., San Millan A., Gutierrez B., Hidalgo L., La Ragione RM., Abuoun M., Galimand M., Ferrandiz MJ., Dominguez L., de la Campa AG. and Gonzalez-Zorn B..


VISAVET PARTICIPANTS


First
José Antonio Escudero García-Calderón
9th
Lucas Domínguez Rodríguez
Last
Bruno González Zorn

DOI: https://doi.org/ 10.1128/AAC.00498-11


CITE THIS PUBLICATION:

Escudero JA., San Millan A., Gutierrez B., Hidalgo L., La Ragione RM., Abuoun M., Galimand M., Ferrandiz MJ., Dominguez L., de la Campa AG. and Gonzalez-Zorn B. Fluoroquinolone efflux in Streptococcus suis is mediated by SatAB and not by SmrA. Antimicrobial Agents and Chemotherapy. 55(12):5850-60. 2011. (A). ISSN: 0066-4804. DOI: 10.1128/AAC.00498-11


UNITS: