Identification of the main quinolone resistance determinant in Campylobacter jejuni and C. coli by MAMA-DEG PCR
Diagnostic Microbiology and Infectious Disease publish this investigation article
March 1st, 2016
Among zoonotic diseases, campylobacteriosis stands out as the major bacterial infection producing human gastroenteritis. Antimicrobial therapy, only recommended in critical cases, is challenged by resistance mechanisms that should be unambiguously detected for achievement of effective treatments. Quinolone (ciprofloxacin) resistance of C. jejuni and C. coli, the two main Campylobacter detected in humans, is conferred by the mutation gyrA C-257-T, which can be genotyped by several methods that require a previous identification of the pathogen species to circumvent the sequence polymorphism of the gene. A multiplex PCR, based on degenerated oligonucleotides, has been designed for unambiguous identification of the quinolone resistance determinant in Campylobacter spp. isolates. The method was verified with 249 Campylobacter strains isolated from humans (141 isolates) and from the three most important animal sources for this zoonosis: poultry (34 isolates), swine (38 isolates) and cattle (36 isolates). High resistance to ciprofloxacin, MIC above 4 g/mL, linked to the mutated genotype predicted by MAMA-DEG PCR was found frequently among isolates from the different hosts.
Hormeno L., Palomo G., Ugarte-Ruiz M., Porrero MC., Borge C., Vadillo S., Piriz S., Dominguez L., Campos MJ. and Quesada A.
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad de Extremadura (UNEX). |
 | Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). |
 | Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad de Córdoba (UCO). |
 | Marine and Environmental Sciences Centre (MARE). Instituto Politécnico de Leiria (IPL). |
| Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Veterinaria. Universidad de Extremadura (UNEX). |
