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Influence of storage conditions on the spectral stability of bacterial suspensions identified by maldi biotyper

Poster presented in 6th Congress of European Microbiologists

June 7th, 2015

Perez-Sancho M., Liandris E., Casamayor A., Pulido E. and Dominguez L.

Although direct smear technique is an excellent approach for the preparation of
samples for MALDI-TOF MS identification, the usefulness of this approach is limited
to BSL-1/BSL-2 bacteria, it is not recommended for MSP creation and it requires
fresh material for an optimal performance. Formic acid extraction is proposed as a
more precise method for MALDI Biotyper identification that includes a step in which
bacteria are suspended in 70% ethanol which could potentially be used for the
storage of bacterial suspensions in their optimal growth phase. However, there is a
lack of information regarding the impact of storage of bacterial suspensions in ethanol on the the reliability of the MALDI-Biotyper identification





Participants:

CEI Campus MoncloaCEI Campus Moncloa.

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).


Link to 6th Congress of European Microbiologists





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6th Congress of European Microbiologists


6th Congress of European Microbiologists
Jun 7th-11st, 2015
Maastricht
The Netherlands

TITLE: Influence of storage conditions on the spectral stability of bacterial suspensions identified by maldi biotyper


TYPE: Poster communication


AUTHORS: Perez-Sancho M., Liandris E., Casamayor A., Pulido E. and Dominguez L.


VISAVET PARTICIPANTS


First
Marta Pérez Sancho
3rd
Almudena Casamayor Sanz
Last
Lucas Domínguez Rodríguez

DATE: June 7th, 2015


CITE THIS COMMUNICATION:

Perez-Sancho M., Liandris E., Casamayor A., Pulido E. and Dominguez L. Influence of storage conditions on the spectral stability of bacterial suspensions identified by maldi biotyper. 6th Congress of European Microbiologists, Federation of European Microbiological Societies, Maastricht, The Netherlands, June 7th, 2015. (Poster communication)


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