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Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain

Frontiers in Microbiology publish this investigation article

June 28th, 2017

Untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. However, its role in the spread of antibiotic resistances in the human population remains poorly investigated. We used whole genome sequencing (WGS) to analyze 25 KPC-2-producing Enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical Citrobacter freundii isolates from a tertiary hospital in the same collection area in Spain. We detected a common, recently described, IncP-6 plasmid carrying the gene blaKPC-2 in 21 isolates from both sources. The plasmid was present in diverse environmental bacterial species of opportunistic pathogens such as C. freundii, Enterobacter cloacae, Klebsiella oxytoca, and Raoultella ornithinolytica. The 40,186 bp IncP-6 plasmid encoded 52 coding sequences (CDS) and was composed of three uniquely combined regions that were derived from other plasmids recently reported in different countries of South America. The region harboring the carbapenem resistance gene (14 kb) contained a Tn3 transposon disrupted by an ISApu-flanked element and the core sequence composed by ISKpn6 / blaKPC-2 / ΔblaTEM-1 / ISKpn27. We document here the presence of a novel promiscuous blaKPC-2 plasmid circulating in environmental bacteria in wastewater and human populations




Yao Y., Lazaro-Perona F., Falgenhauer L., Valverde A., Imizalioglu C., Dominguez L., Canton R., Mingorance J. and Chakraborty T.




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Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain

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Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain


Participants:

Justus-Liebig-Universität GießenInstitut für Medizinische Mikrobiologie. Justus-Liebig-Universität Gießen (JLU).

German Center for InfectionPartner Site Giessen-Marburg-Langen. German Center for Infection (DZIF).

Comunidad de MadridServicio de Microbiología. Hospital Universitario La Paz (HPAZ). Salud Madrid. Comunidad de Madrid.

Comunidad de MadridInstituto de Investigación Hospital La Paz (IdiPAZ). Consejería de Sanidad. Comunidad de Madrid.

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Instituto de Salud Carlos IIIRed Española de Investigación en Patología Infecciosa (REIPI). Instituto de Salud Carlos III (ISCIII).

Comunidad de MadridServicio de Microbiología. Hospital Universitario Ramón y Cajal. Salud Madrid. Comunidad de Madrid.

Comunidad de MadridInstituto Ramón y Cajal de Investigación Sanitaria (IRYCIS). Universidad Complutense (UCM). Universidad Autónoma de Madrid (UAM). Universidad de Alcalá (UAH). Salud Madrid. Comunidad de Madrid.







Frontiers in Microbiology
FACTOR YEAR Q
4.109 2017

NLMID: 101548977

PMID: 28702005

ISSN: 1664-302X



TITLE: Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain


JOURNAL: Front Microbiol


NUMERACIÓN: 8:1143


AÑO: 2017


PUBLISHER: Frontiers Research Foundation


AUTHORS: Yao Y., Lazaro-Perona F., Falgenhauer L., Valverde A., Imizalioglu C., Dominguez L., Canton R., Mingorance J. and Chakraborty T.


VISAVET PARTICIPANTS


Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.3389/fmicb.2017.01143


CITE THIS PUBLICATION:

Yao Y., Lazaro-Perona F., Falgenhauer L., Valverde A., Imizalioglu C., Dominguez L., Canton R., Mingorance J. and Chakraborty T. Insights into a novel blaKPC-2 -encoding IncP-6 plasmid reveal carbapenem-resistance circulation in several Enterobacteriaceae species from wastewater and a hospital source in Spain. Frontiers in Microbiology. 8:1143. 2017. (A). ISSN: 1664-302X. DOI: 10.3389/fmicb.2017.01143


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