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Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains

Investigation published in Veterinary Immunology and Immunopathology

July 15th, 2014

Antibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. In contrast, information on the cellular mediated immune (CMI) response triggered after Brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced CMI reaction. Here, the interferon-gamma (IFN-γ) profiles evoked after exposure by different routes to virulent (H38) and attenuated (Rev.1) B. melitensis strains in 14 pregnant sheep and 87 ewe lambs, respectively, were characterized accounting for different host- related factors, and compared with their serological response and with the basal IFN- γ responses observed in 155 animals non exposed to Brucella. No significant differences in the IFN- γ response of Rev.1 vaccinated animals depending on the inoculation route was observed, in contrast with their serological results. Response in H38-challenged followed a similar trend although peaked later, and an effect of the abortion on the IFN-γ response was detected. This information could help to understand the interaction bacteria-host that leads to its intracellular survival and could be useful for the design of new diagnostic approaches




Perez-Sancho M., Duran-Ferrer M., Garcia-Seco T., Macias P., Garcia N., Martinez I., Ruiz E., Legaz E., Diez-Guerrier A., Gonzalez S., Dominguez L. and Alvarez J..




See this article
Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains

See it on NLM PubMed
Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains


Participants:

Ministerio de Agricultura, Alimentación y Medio AmbienteLaboratorio Central de Sanidad Animal (LCSA). Área de Ganadería. Ministerio de Agricultura, Alimentación y Medio Ambiente (MAGRAMA).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

University of MinnesotaCollege of Veterinary Medicine. University of Minnesota (UMM).

Castellana de Ganaderos Sociedad Cooperativa.







Veterinary Immunology and Immunopathology
FACTOR YEAR Q
1.535 2014

NLMID: 8002006

PMID: 24814244

ISSN: 0165-2427



TITLE: Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains


JOURNAL: Vet. Immunol. Immunopathol.


NUMERACIÓN: 160(1-2):123-8


AÑO: 2014


PUBLISHER: Elsevier Scientific


AUTHORS: Perez-Sancho M., Duran-Ferrer M., Garcia-Seco T., Macias P., Garcia N., Martinez I., Ruiz E., Legaz E., Diez-Guerrier A., Gonzalez S., Dominguez L. and Alvarez J..


VISAVET PARTICIPANTS


First
Marta Pérez Sancho
3rd
Teresa García-Seco Romero
5th
Nerea García Benzaquén
6th
Irene Martínez Alares
9th
Alberto Antoine Díez Guerrier
10
Sergio González Domínguez
11th
Lucas Domínguez Rodríguez
Last
Julio Álvarez Sánchez

DOI: https://doi.org/ 10.1016/j.vetimm.2014.03.014


CITE THIS PUBLICATION:

Perez-Sancho M., Duran-Ferrer M., Garcia-Seco T., Macias P., Garcia N., Martinez I., Ruiz E., Legaz E., Diez-Guerrier A., Gonzalez S., Dominguez L. and Alvarez J. Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains. Veterinary Immunology and Immunopathology. 160(1-2):123-8. 2014. (A). ISSN: 0165-2427. DOI: 10.1016/j.vetimm.2014.03.014


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