Porcine Health Management publish this investigation article

March 17^th, 2022

Background
Resistance to colistin was an uncommon phenomenon traditionally linked to chromosome point mutations, but since the first description of a plasmid-mediated colistin-resistance in late 2015, transmissible resistance to colistin has become a Public Health concern. Despite colistin is considered as a human last resort antibiotic, it has been commonly used in swine industry to treat post-weaning diarrhoea in piglets. However, the progressively increase of colistin resistance during the last decade led to the Spanish Medicines and Healthcare Products Agency (AEMPS) to launch a strategic and voluntary plan aimed to reduce colistin consumption in pig production. Our longitudinal study (1998-2021) aimed to evaluate the trend of colistin resistance mediated through the mcr-1 mobile gene in Spanish food-producing pig population and compare it with published polymyxin sales data in veterinary medicine to assess their possible relationships.
Results
The first mcr-1 positive sample was observed in 2004, as all samples from 1998 and 2002 were mcr-1 PCR-negative. We observed a progressive increase of positive samples from 2004 to 2015, when mcr-1 detection reached its maximum peak (33/50; 66%). From 2017 (27/50; 54%) to 2021 (14/81; 17%) the trend became downward, reaching percentages significantly lower than the 2015 peak (p < 0.001). The abundance of mcr-1 gene in PCR-positive samples showed a similar trend reaching the highest levels in 2015 (median: 6.6×10 4 mcr-1 copies/mg of faeces), but decreased significantly from 2017 to 2019 (median 2.7×10 4 , 1.2×10 3 , 4.6×10 2
mcr-1 copies/mg of faeces for 2017, 2018 and 2019, respectively), and stabilizing in 2021 (1.6×10 2 mcr-1 copies/mg of faeces) with similar values than 2019.
Conclusions
Our study showed the decreasing trend of colistin resistance associated to mcr-1 gene, after a previous increase from among 2004-2015, since the European Medicines Agency and AEMPS strategies were applied in 2016 to reduce colistin use in animals, suggesting a connection between polymyxin use and colistin resistance. Thus, these plans could have been effective in mcr-1 reduction, reaching lower levels than those detected in samples collected 17 years ago, when resistance to colistin was not yet a major concern

Miguela-Villoldo P., Moreno MA., Rodriguez-Lazaro D., Gallardo A., Hernandez M., Serrano T., Saez-Llorente JL., De Frutos C., Aguero M., Quesada A., Dominguez L. and Ugarte-Ruiz M..

Longitudinal study of the mcr-1 gene prevalence in Spanish food-producing pigs from 1998 to 2021 and its relationship with the use of polymyxins

Longitudinal study of the mcr-1 gene prevalence in Spanish food-producing pigs from 1998 to 2021 and its relationship with the use of polymyxins

	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Unidad de Microbiología. Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos (UBU).
	Departamento de Bioquímica, Biología Molecular y Genética. Facultad de Veterinaria. Universidad de Extremadura (UNEX).
	Laboratorio de Biología Molecular y Microbiología. Instituto Tecnológico Agrario de Castilla y León (ITACyL).
	Tecnologías y Servicios Agrarios S.A. TRAGSATEC.
	Subdirección General de Sanidad e Higiene Animal y Trazabilidad. Dirección General de la Producción Agraria. Ministerio de Agricultura, Pesca y Alimentación (MAPA).
	Laboratorio Central de Veterinaria (LCV). Ministerio de Agricultura, Pesca y Alimentación (MAPA).
	Instituto Universitario de Biotecnología Ganadera y Cinegética (INBIO G+C). Universidad de Extremadura (UNEX).

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- Un estudio refleja el descenso de la resistencia a la colistina en los cerdos de España - animalshealth.es