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Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates

Investigation article published in Applied and Environmental Microbiology

December 1st, 2001

A total of 153 strains of Listeria monocytogenes isolated from different sources (72 from sheep, 12 from cattle, 18 from feedstuffs, and 51 from humans) in Spain from 1989 to 2000 were characterized by pulsed-field gel electrophoresis. The strains of L. monocytogenes displayed 55 pulsotypes. The 84 animal, 51 human, and 18 feedstuff strains displayed 31, 29, and 7 different pulsotypes, respectively, indicating a great genetic diversity among the Spanish L. monocytogenes isolates studied. L. monocytogenes isolates from clinical samples and feedstuffs consumed by the diseased animals were analyzed in 21 flocks. In most cases, clinical strains from different animals of the same flock had identical pulsotypes, confirming the existence of a listeriosis outbreak. L. monocytogenes strains with pulsotypes identical to those of clinical strains were isolated from silage, potatoes, and maize stalks. This is the first study wherein potatoes and maize stalks are epidemiologically linked with clinical listeriosis




Vela AI., Fernandez-Garayzabal JF., Vazquez-Boland JA., Latre MV., Blanco MM., Moreno MA., de la Fuente I., Marco J., Franco CM., Cepeda A., Rodriguez AA., Suarez G. and Dominguez L..




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Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates

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Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Applied and Environmental Microbiology
FACTOR YEAR Q
3.688 2001

NLMID: 7605801

PMID: 11722943

ISSN: 0099-2240



TITLE: Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates


JOURNAL: Appl Environ Microbiol


NUMERACIÓN: 67(12):5840-3


AÑO: 2001


PUBLISHER: American Society for Microbiology


AUTHORS: Vela AI., Fernandez-Garayzabal JF., Vazquez-Boland JA., Latre MV., Blanco MM., Moreno MA., de la Fuente I., Marco J., Franco CM., Cepeda A., Rodriguez AA., Suarez G. and Dominguez L..


First
Ana Isabel Vela Alonso
2nd
José Francisco Fernández-Garayzabal Fernández
Last
Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1128/AEM.67.12.5840-5843.2001


CITE THIS PUBLICATION:

Vela AI., Fernandez-Garayzabal JF., Vazquez-Boland JA., Latre MV., Blanco MM., Moreno MA., de la Fuente I., Marco J., Franco CM., Cepeda A., Rodriguez AA., Suarez G. and Dominguez L. Molecular typing by pulsed-field gel electrophoresis of Spanish animal and human Listeria monocytogenes isolates. Applied and Environmental Microbiology. 67(12):5840-3. 2001. (A). ISSN: 0099-2240. DOI: 10.1128/AEM.67.12.5840-5843.2001


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