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Multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by M. bovis in cattle

Investigation published in Veterinary Microbiology

January 30th, 2015

The combination of new genotyping tools and a more exhaustive sampling policy in the analysis of infection by Mycobacterium tuberculosis has shown that infection by this pathogen is more complex than initially expected. Mixed infections, coexistence of clonal variants from a parental strain, and compartmentalized infections are all different modalities of this clonal complexity. Until recently, genotyping of Mycobacterium bovis in animal populations was based on spoligotyping and analysis of a single isolate per infection; therefore, clonal complexity is probably underdetected. We used multiple sampling combined with highly discriminatory MIRU-VNTR to study compartmentalized infections by M. bovis in a low-tuberculosis prevalence setting. We spoligotyped the M. bovis isolates from two or more anatomic locations sampled from 55 animals on 39 independent farms. Compartmentalized infections, with two different strains infecting independent lymph nodes in the same animal, were found in six cases (10.9%). MIRU-VNTR analysis confirmed that the compartmentalization was strict and that only one strain was present in each infected node. MIRU-VNTR analysis of additional infected animals on one of the farms confirmed that the compartmentalized infection was a consequence of superinfection, since the two strains were independently infecting other animals. This same analysis revealed the emergence of a microevolved clonal variant in one of the lymph nodes of the compartmentalized animal. Clonal complexity must also be taken into consideration in M. bovis infection, even in low-prevalence settings, and analyses must be adapted to detect it and increase the accuracy of molecular epidemiology studies




Navarro Y., Romero B., Copano MF., Bouza E., Dominguez L., de Juan L. and Garcia de Viedma D.




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Multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by M. bovis in cattle

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Multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by M. bovis in cattle



Participants:

Comunidad de MadridServicio de Microbiología y Enfermedades Infecciosas. Hospital General Universitario Gregorio Marañón. Consejería de Sanidad. Comunidad de Madrid.

Instituto de Salud Carlos IIICentro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES). Instituto de Salud Carlos III (ISCIII).

CEI Campus MoncloaCEI Campus Moncloa.

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Gobierno del Principado de AsturiasLaboratorio de Sanidad Animal. Gobierno del Principado de Asturias.

Universidad ComplutenseFacultad de Medicina. Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
2.564 2015

NLMID: 7705469

PMID: 25439651

ISSN: 0378-1135



TITLE: Multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by M. bovis in cattle


JOURNAL: Vet. Microbiol.


NUMERACIÓN: 175(1):99-104


AÑO: 2015


PUBLISHER: Elsevier Science Publishers


AUTHORS: Navarro Y., Romero B., Copano MF., Bouza E., Dominguez L., de Juan L. and Garcia de Viedma D.


VISAVET PARTICIPANTS


2nd
Beatriz Romero Martínez
5th
Lucas Domínguez Rodríguez
6th
Lucía de Juan Ferré

DOI: https://doi.org/10.1016/j.vetmic.2014.11.004


CITE THIS PUBLICATION:

Navarro Y., Romero B., Copano MF., Bouza E., Dominguez L., de Juan L. and Garcia de Viedma D. Multiple sampling and discriminatory fingerprinting reveals clonally complex and compartmentalized infections by M. bovis in cattle. Veterinary Microbiology. 175(1):99-104. 2015. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2014.11.004


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