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Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish

Investigation article published in Applied and Environmental Microbiology

May 1st, 2004

A multiplex PCR-based method was designed for the simultaneous detection of the main pathogens involved in warm-water streptococcosis in fish (Streptococcus iniae, Streptococcus difficilis, Streptococcus parauberis, and Lactococcus garvieae). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. The sensitivity of the multiplex PCR using purified DNA was 25 pg for S. iniae, 12.5 pg for S. difficilis, 50 pg for S. parauberis, and 30 pg for L. garvieae. The multiplex PCR assay was useful for the specific detection of the four species of bacteria not only in pure culture but also in inoculated fish tissue homogenates and naturally infected fish. Therefore, this method could be a useful alternative to the culture-based method for the routine diagnosis of warm-water streptococcal infections in fish




Mata AI., Gibello A., Casamayor A., Blanco MM., Dominguez L. and Fernandez-Garayzabal JF..




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Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish

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Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







Applied and Environmental Microbiology
FACTOR YEAR Q
3.810 2004

NLMID: 7605801

PMID: 15128589

ISSN: 0099-2240



TITLE: Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish


JOURNAL: Appl Environ Microbiol


NUMERACIÓN: 70(5):3183-7


AÑO: 2004


PUBLISHER: American Society for Microbiology


AUTHORS: Mata AI., Gibello A., Casamayor A., Blanco MM., Dominguez L. and Fernandez-Garayzabal JF..


3rd
Almudena Casamayor Sanz
5th
Lucas Domínguez Rodríguez
Last
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1128/AEM.70.5.3183-3187.2004


CITE THIS PUBLICATION:

Mata AI., Gibello A., Casamayor A., Blanco MM., Dominguez L. and Fernandez-Garayzabal JF. Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish. Applied and Environmental Microbiology. 70(5):3183-7. 2004. (A). ISSN: 0099-2240. DOI: 10.1128/AEM.70.5.3183-3187.2004


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