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Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids

Antimicrobial Agents and Chemotherapy publish this investigation article

August 1st, 2009

In most gram-negative bacteria, acquired multiresistance is conferred by large plasmids compiling numerous antimicrobial resistance genes. Here, we show an evolutionary alternative strategy used by Pasteurella multocida to become resistant to multiple clinically relevant antibiotics. Thirteen beta-lactam-resistant clinical isolates, concomitantly resistant to tetracyclines and/or streptomycin as well as to sulfonamides, were studied. Pulsed-field gel electrophoresis analysis revealed different profiles among the isolates, showing that clonal dissemination was not the sole event responsible for the spread of multiresistance. Each P. multocida strain carried two or three small plasmids between 4 and 6 kb in size. A direct association between resistance profile and plasmid content was found. Complete nucleotide sequencing of all plasmids revealed seven different replicons, six of them belonging to the ColE1 superfamily. All plasmids carried one, or a maximum of two, antimicrobial resistance determinants. Plasmids pB1000 and pB1002 bore bla(ROB-1), pB1001 carried tet(B), pB1003 and pB1005 carried sul2 and strA, pB1006 harbored tet(O), and p9956 bore the tet(H) gene. All plasmids except pB1002 and pB1006 were successfully transformed into Escherichia coli. pB1000, also involved in beta-lactam resistance in Haemophilus parasuis (A. San Millan et al., Antimicrob. Agents Chemother. 51:2260-2264, 2007), was mobilized in E. coli using the conjugation machinery of an IncP plasmid. Stability experiments proved that pB1000 was stable in P. multocida but highly unstable in E. coli. In conclusion, bla(ROB-1) is responsible for beta-lactam resistance in P. multocida in Spain. Coexistence and the spread of small plasmids are used by P. multocida to become multiresistant




San Millan A., Escudero JA., Gutierrez B., Hidalgo L., Garcia N., Llagostera M., Dominguez L. and Gonzalez-Zorn B..




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Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids

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Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids



Participants:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universitat Autònoma de BarcelonaDepartament de Genètica i de Microbiologia. Universitat Autònoma de Barcelona (UAB).

Universitat Autònoma de BarcelonaCentre de Recerca en Sanitat Animal (CReSA). Universitat Autònoma de Barcelona (UAB).

Generalitat de CatalunyaInstituto de Investigación y Tecnología Agroalimentarias (IRTA). Generalitat de Catalunya.







Antimicrobial Agents and Chemotherapy
FACTOR YEAR Q
4.802 2009

NLMID: 315061

PMID: 19528282

ISSN: 0066-4804



TITLE: Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids


JOURNAL: Antimicrob. Agents Chemother.


NUMERACIÓN: 53(8):3399-404


AÑO: 2009


PUBLISHER: American Society for Microbiology


AUTHORS: San Millan A., Escudero JA., Gutierrez B., Hidalgo L., Garcia N., Llagostera M., Dominguez L. and Gonzalez-Zorn B..


VISAVET PARTICIPANTS


2nd
José Antonio Escudero García-Calderón
5th
Nerea García Benzaquén
7th
Lucas Domínguez Rodríguez
Last
Bruno González Zorn

DOI: https://doi.org/10.1128/AAC.01522-08


CITE THIS PUBLICATION:

San Millan A., Escudero JA., Gutierrez B., Hidalgo L., Garcia N., Llagostera M., Dominguez L. and Gonzalez-Zorn B. Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids. Antimicrobial Agents and Chemotherapy. 53(8):3399-404. 2009. (A). ISSN: 0066-4804. DOI: 10.1128/AAC.01522-08


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