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New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers

Investigation article published in Veterinary Medicine and Science

January 18th, 2019

European badgers (Meles meles) have been identified as wildlife reservoirs for Mycobacterium bovis in the UK and Ireland, and may also have a role in the epidemiology of animal tuberculosis in other European regions. Thus, detection of M. bovis-infected badgers may be required for the purposes of surveillance and monitoring of disease levels in infected populations. Current serological assays to detect M. bovis infection in live badgers, while rapid and inexpensive, show limited diagnostic sensitivity. Here we describe and evaluate new ELISA platforms for the recognition of the P22 multiprotein complex derived from the purified protein derivative (PPD) of M. bovis. The recognition of IgG against P22 multiprotein complex derived from PPD-B was tested by ELISA in the serum of badgers from the UK, Ireland and Spain. TB infection in the badgers was indicated by the presence of M. bovis in tissues by culture and histology at post-mortem examination and TB-free status was established by repeated negativity in the interferon γ release assay (IGRA). In experimentally infected badgers, humoral antibody responses against P22 developed within 45 days post-infection. The ELISA tests showed estimated sensitivity levels of 74-82% in experimentally and naturally infected badgers with specificities ranging from 75% to 100% depending on the badger population tested. The P22 multi-antigen based ELISAs provide a sensitive and specific test platform for improved tuberculosis surveillance in badgers




Infantes-Lorenzo JA., Dave D., Moreno I., Anderson P., Lesellier S., Gormley E., Dominguez L., Balseiro A., Gortazar C., Dominguez M. and Salguero FJ.




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New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers

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New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers

Participants:

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Animal and Plant Health AgencyDepartment of Bacteriology. Animal and Plant Health Agency (APHA).

Instituto de Salud Carlos IIIServicio de Inmunología. Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

University College DublinUCD School of Agriculture, Food Science & Veterinary Medicine. University College Dublin (UCD).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Gobierno del Principado de AsturiasCentro de Biotecnología Animal. Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA). Gobierno del Principado de Asturias.

Gobierno de Castilla-La ManchaSanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).

University of SurreySchool of Veterinary Medicine. Faculty of Health and Medical Sciences. University of Surrey.







FACTOR YEAR Q
0.943 2018

PMID: 30656864

ISSN: 2053-1095



TITLE: New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers


JOURNAL: Vet. Med. Sci.


NUMERACIÓN: 5(1):61-69


AÑO: 2019


PUBLISHER: American Veterinary Medical Association


AUTHORS: Infantes-Lorenzo JA., Dave D., Moreno I., Anderson P., Lesellier S., Gormley E., Dominguez L., Balseiro A., Gortazar C., Dominguez M. and Salguero FJ.


VISAVET PARTICIPANTS


First
Jose Antonio Infantes Lorenzo
7th
Lucas Domínguez Rodríguez

DOI: https://doi.org/ 10.1002/vms3.134


CITE THIS PUBLICATION:

Infantes-Lorenzo JA., Dave D., Moreno I., Anderson P., Lesellier S., Gormley E., Dominguez L., Balseiro A., Gortazar C., Dominguez M. and Salguero FJ. New serological platform for detecting antibodies against Mycobacterium tuberculosis complex in European badgers. Veterinary Medicine and Science. 5(1):61-69. 2019. (A). ISSN: 2053-1095. DOI: 10.1002/vms3.134


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