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Occurrence of Hepatitis E virus in pigs, pork cuts and organs at the Time of Slaughter, Spain, 2017

Investigation published in Frontiers in Microbiology

In Press

Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver-derivates are considered the major risk products, and studies focused on the presence of HEV in pig muscles are scarce. The objective of the present study was to evaluate the presence of HEV in different organs and tissues of 45 apparently healthy pigs from 9 Spanish slaughterhouses (50% National production), that could enter into the food supply chain. Anti-HEV antibodies were evaluated in serum by an ELISA test. Ten samples from each animal were analyzed for the presence of HEV RNA by a RT-qPCR. The overall seroprevalence obtained was 73.3% (33/45). From the 450 samples analyzed a total of 26 RT-qPCR positive samples were identified in liver (7/45), faeces (6/45), kidney (5/45), heart (4/45), serum (3/45), and diaphragm (1/45). This is the first report on detection of HEV RNA in kidney and heart samples of naturally infected pigs. HEV RNA detection was negative for rib, bacon, lean ham and loin samples. These findings indicate that pig meat could be considered as a low risk material for foodborne HEV infection




Garcia N., Hernandez M., Gutierrez-Boada M., Valero A., Navarro A., Munoz-Chimeno M., Fernandez-Manzano A., Escobar FM., Martinez I., Barcena C., Gonzalez S., Avellon A., Eiros JM., Fongaro G., Dominguez L., Goyache J. and Rodriguez-Lazaro D.




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Occurrence of Hepatitis E virus in pigs, pork cuts and organs at the Time of Slaughter, Spain, 2017




Participants:

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad de BurgosUnidad de Microbiología. Departamento de Biotecnología y Ciencia de los Alimentos. Universidad de Burgos (UBU).

Instituto Tecnológico Agrario de Castilla y LeónInstituto Tecnológico Agrario de Castilla y León (ITACyL).

Universidad de CórdobaUniversidad de Córdoba (UCO).

Universidade Federal de Santa CatarinaUniversidade Federal de Santa Catarina (UFSC).

Hospital Universitario Río Hortega de ValladolidHospital Universitario Río Hortega de Valladolid.

Ministerio de Sanidad, Consumo y Bienestar SocialSistema Nacional de Salud (SNS). Ministerio de Sanidad, Consumo y Bienestar Social (MSCBS).

National University of Río CuartoNational University of Río Cuarto (UNRC).







Frontiers in Microbiology
FACTOR YEAR Q
4.259 2018

NLMID: 101548977

ISSN: 1664-302X



TITLE: Occurrence of Hepatitis E virus in pigs, pork cuts and organs at the Time of Slaughter, Spain, 2017


JOURNAL: Front Microbiol


PUBLISHER: Frontiers Research Foundation


AUTHORS: Garcia N., Hernandez M., Gutierrez-Boada M., Valero A., Navarro A., Munoz-Chimeno M., Fernandez-Manzano A., Escobar FM., Martinez I., Barcena C., Gonzalez S., Avellon A., Eiros JM., Fongaro G., Dominguez L., Goyache J. and Rodriguez-Lazaro D.


VISAVET PARTICIPANTS


First
Nerea García Benzaquén
5th
Alejandro Navarro Gómez
7th
Álvaro Fernández Manzano
9th
Irene Martínez Alares
10
Carmen Bárcena Asensio
11th
Sergio González Domínguez
15th
Lucas Domínguez Rodríguez
16th
Joaquín Goyache Goñi

DOI: https://doi.org/ 10.3389/fmicb.2019.02990


CITE THIS PUBLICATION:

Garcia N., Hernandez M., Gutierrez-Boada M., Valero A., Navarro A., Munoz-Chimeno M., Fernandez-Manzano A., Escobar FM., Martinez I., Barcena C., Gonzalez S., Avellon A., Eiros JM., Fongaro G., Dominguez L., Goyache J. and Rodriguez-Lazaro D. Occurrence of Hepatitis E virus in pigs, pork cuts and organs at the Time of Slaughter, Spain, 2017. Frontiers in Microbiology. In Press. (A). ISSN: 1664-302X. DOI: 10.3389/fmicb.2019.02990


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