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Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles

Investigation published in Journal of Veterinary Diagnostic Investigation

November 1st, 2018

Staphylococcus aureus encompasses 2 subspecies ( aureus and anaerobius) with significant differences in their epidemiology and pathogenicity. We evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the rapid identification of both subspecies using a panel of 52 S. aureus isolates (30 subsp. anaerobius and 22 subsp. aureus) recovered from different origins, countries, and years. The on-board library identification system correctly identified 42 of 52 (81%) S. aureus isolates at the species level with score values >2.0. Limited performance was observed for differentiation of S. aureus subspecies (particularly subsp. anaerobius). Visual inspection of MALDI-TOF MS profiles identified 5 subspecies-specific mass peaks ( m/ z 3430 and 6861 in S. aureus subsp. anaerobius, and m/ z 4046, 6890, and 8093 in S. aureus subsp. aureus) with 100% sensitivity and specificity values, which is potentially useful for differentiating these subspecies. The suitability of 3 models, Genetic Analysis (GA), Quick Classifier (QC), and Supervised Neural Network, for automatic identification of both subspecies was evaluated using the Recognition Capability (RC) and Cross Validation (CV) values provided by the on-board ClinProTools software. The GA and QC models reached RC and CV values of 100%. Both models were externally validated using a panel of 26 S. aureus isolates of both subspecies, with both models correctly classifying all isolates of both subspecies. MALDI-TOF MS coupled with ClinProTools software represents a rapid and simple approach for S. aureus subspecies discrimination




Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. and de la Fuente R.




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Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles

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Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles



Participants:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Journal of Veterinary Diagnostic Investigation
FACTOR YEAR Q
1.174 2018

NLMID: 9011490

PMID: 30280650

ISSN: 1040-6387



TITLE: Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles


JOURNAL: J Vet Diagn Invest


NUMERACIÓN: 30(6):813-820


AÑO: 2018


PUBLISHER: American Association Of Veterinary Laboratory Diagnosticians


AUTHORS: Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. and de la Fuente R.


VISAVET PARTICIPANTS


First
Marta Pérez Sancho
2nd
Ana Isabel Vela Alonso
4th
María Ugarte Ruiz
5th
Lucas Domínguez Rodríguez
6th
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/ 10.1177/1040638718805537


CITE THIS PUBLICATION:

Perez-Sancho M., Vela AI., Horcajo P., Ugarte-Ruiz M., Dominguez L., Fernandez-Garayzabal JF. and de la Fuente R. Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles. Journal of Veterinary Diagnostic Investigation . 30(6):813-820. 2018. (A). ISSN: 1040-6387. DOI: 10.1177/1040638718805537


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