Poster presented in Med-Vet-Net Association International Scientific Conference 2013 (One health, one medicine: sharing challenges for combating zoonoses)

June 24^th, 2013

Porrero MC., Mentaberre G., Sanchez S., Fernandez-Llario P., Gomez S., Navarro-Gonzalez N., Serrano E., Casas-Diaz E., Marco I., Fernandez-Garayzabal JF., Mateos A., Vidal D., Lavin S. and Dominguez L.

Staphylococcus aureus is considered a commensal bacterium in animals and humans, but S. aureus carriage in wild animals has not been analysed. Swabs from skin and nasal samples (nares) were taken from Eurasian griffon vulture (n=40 samples/ 40 animals), Iberian ibex
(n=260 samples /157 animals), mouflon (n=4 samples/2 animals), red deer (n=544 samples/ 273 animals) and wild boar (n=1395 samples/716 animals) and cultured on Baird-Parker agar with rabbit plasma fibrinogen;compatible colonies (one per sample) were confirmed by PCR. The presence of mecA and mecC genes and spa typing were performed in all isolates. In total, 247 isolates from 226 animals were recovered, the most of them from nasal samples (77.23%). Two isolates were mecA positive (0.008%). Sixty five spa types (including 27 new descriptions) were detected, the most frequent ones being t3750
(n=53), t548 (n=27) and t528 (n=18).Several spa types were mainly isolated from a particular animal species (98.1% and 94.4% of spa type t3750 and t528 isolates were from wild boars and Iberian ibex,
respectively; the 66.7% of isolates spa type t548 were from wild boar and 33.3% from red deer). These data suggested that some spa types could be host adapted. Both mecA-carrying isolates (t127 and t011) were from wild boars. Although the protocol applied was not specific for the detection of MRSA, this result points out the interest of monitoring MRSA in wildlife. Moreover, the detected MRSA genotypes are frequently associated with MRSA from livestock and humans, which could indicate contacts between different reservoirs. Further studies regarding bacterial dissemination are to be evaluated, including water as route of spreading from human and domestic habitats to wildlife

	Servicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).