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Streptococcus equi subsp. ruminatorum subsp. nov., isolated from mastitis in small ruminants

Investigation published in International Journal of Systematic and Evolutionary Microbiology

November 1st, 2004

Six isolates of an unknown Gram-positive, catalase-negative, chain-forming, coccus-shaped organism isolated from ovine and caprine mastitis were characterized by phenotypic and molecular taxonomic methods. On the basis of cellular morphology and the results of biochemical tests, the organism was tentatively identified as a streptococcal species. Comparative 16S rRNA gene sequencing studies confirmed that the organism is a member of the genus Streptococcus, with Streptococcus equi as its closest phylogenetic relative (98.8 % similarity). DNA-DNA pairing studies showed that the unidentified organism displayed more than 70 % relatedness to the type strains of S. equi subsp. equi and subsp. zooepidemicus. Despite the relatively high DNA-DNA reassociation values, biotyping and ribotyping allowed clear differentiation of the unknown bacterium from the two recognized subspecies of S. equi. On the basis of phenotypic and molecular genetic evidence, it is proposed that the unknown Streptococcus isolates from ovine and caprine mastitis be classified as a novel subspecies, Streptococcus equi subsp. ruminatorum subsp. nov. The type strain is CECT 5772(T) (=CCUG 47520(T)=Mt 167(T)).




Fernandez E., Blume V., Garrido P., Collins MD., Mateos A., Dominguez L. and Fernandez-Garayzabal JF..




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Streptococcus equi subsp. ruminatorum subsp. nov., isolated from mastitis in small ruminants

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Streptococcus equi subsp. ruminatorum subsp. nov., isolated from mastitis in small ruminants



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

School of Food Biosciences. University of Reading.







International Journal of Systematic and Evolutionary Microbiology
FACTOR YEAR Q
3.015 2004

NLMID: 100899600

PMID: 15545473

ISSN: 1466-5026



TITLE: Streptococcus equi subsp. ruminatorum subsp. nov., isolated from mastitis in small ruminants


JOURNAL: Int J Syst Evol Microbiol


NUMERACIÓN: 54(Pt 6):2291-6


AÑO: 2004


PUBLISHER: Society for General Microbiology


AUTHORS: Fernandez E., Blume V., Garrido P., Collins MD., Mateos A., Dominguez L. and Fernandez-Garayzabal JF..


6th
Lucas Domínguez Rodríguez
Last
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1099/ijs.0.63145-0


CITE THIS PUBLICATION:

Fernandez E., Blume V., Garrido P., Collins MD., Mateos A., Dominguez L. and Fernandez-Garayzabal JF. Streptococcus equi subsp. ruminatorum subsp. nov., isolated from mastitis in small ruminants. International Journal of Systematic and Evolutionary Microbiology. 54(Pt 6):2291-6. 2004. (A). ISSN: 1466-5026. DOI: 10.1099/ijs.0.63145-0


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