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The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain

Veterinary Microbiology publish this investigation article

March 1st, 1997

A total of 129 Mycobacterium bovis strains from 5 different Spanish locations were fingerprinted using the IS6110 repetitive element. We demonstrated the presence of multiple copies (from 2 to 13) of IS6110 in a large proportion (47.4%) of the M.bovis strains isolated from cattle and we showed that these strains can be successfully differentiated by means of the RFLP with IS6110. All of the M. bovis strains isolated from goats had multiple copies of IS6110 and 4 bands of 2, 1.7, 1.4 and 1.3 kb were common in all the caprine RFLP patterns. The caprine strains formed a clearly separate cluster from the bovine strains




Liebana E., Aranaz A., Dominguez L., Mateos A., Gonzalez-Llamazares O., Rodriguez-Ferri E., Domingo M., Vidal D. and Cousins DV.




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The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain

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The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad de LeónDepartamento de Patología Animal, Medicina Animal (Anatomía Patológica). Facultad de Veterinaria. Universidad de León.

Universitat Autònoma de BarcelonaDepartamento de Patología y Producciones Animales. Facultad de Veterinaria. Universitat Autònoma de Barcelona (UAB).

Department of Agriculture and Food Western AustraliaLaboratory for Bovine Tuberculosis. Animal Health Laboratories (AHL). Department of Agriculture and Food Western Australia (DAFWA).







Veterinary Microbiology
FACTOR YEAR Q
1.251 1997

NLMID: 7705469

PMID: 9100324

ISSN: 0378-1135



TITLE: The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain


JOURNAL: Vet Microbiol


NUMERACIÓN: 54(3-4):223-33


AÑO: 1997


PUBLISHER: Elsevier


AUTHORS: Liebana E., Aranaz A., Dominguez L., Mateos A., Gonzalez-Llamazares O., Rodriguez-Ferri E., Domingo M., Vidal D. and Cousins DV.


Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1016/S0378-1135(96)01282-5


CITE THIS PUBLICATION:

Liebana E., Aranaz A., Dominguez L., Mateos A., Gonzalez-Llamazares O., Rodriguez-Ferri E., Domingo M., Vidal D. and Cousins DV. The insertion element IS 6110 is a useful tool for DNA fingerprinting of Mycobacterium bovis isolates from cattle and goats in Spain. Veterinary Microbiology. 54(3-4):223-33. 1997. (A). ISSN: 0378-1135. DOI: 10.1016/S0378-1135(96)01282-5


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