Investigation article published in Veterinary Microbiology

September 1^st, 2017

Deer species (family Cervidae) are often part of the Mycobacterium tuberculosis complex maintenance host community, and tuberculosis (TB) control in deer, including vaccination, is consequently an area of ongoing research. However, most research into deer vaccination against TB is focused on using the live bacillus Calmette Guerin (BCG). Oral inactivated vaccines represent an interesting alternative to either oral or parenteral BCG, since neither diagnostic cross-reactions nor vaccine strain survival are likely to occur. In order to describe the red deer response to heat-inactivated M. bovis (IV) as compared to BCG and to unvaccinated controls (n = 5/group), we ran an experiment with five month-old vaccinated red deer, which were challenged with a virulent M. bovis strain 70 days later and necropsied at 60 days post-challenge. A reduction in the IV group infection burden was discovered. There were significant differences between the IV group and the control group (53% lesion reduction) as regards to the TB lesion scores, but not between other pairs. Complement component 3 plasma levels increased after challenge, and there were no differences between groups. The plasma cytokines (IL-1β, TNFα, IFNγ, IL-10 and IL-12) levels did not change after vaccination, but IL-1β, TNFα and IL-10 did so following the challenge. The IL-1β level increased in all the groups while TNFα levels had a distinct response pattern in the IV group and IL-10 had a distinct response pattern in control group. The results showed that oral vaccination with IV reduces the TB lesion score in red deer challenged with a M. bovis field strain without interfering with the in vivo diagnosis of infection in this species. © 2017 Elsevier B.V.

Thomas J., Risalde MA., Serrano M., Sevilla I., Geijo M., Ortiz JA., Fuertes M., Ruiz-Fons JF., de la Fuente J., Dominguez L., Juste R., Garrido J. and Gortazar C.

The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain

The response of red deer to oral administration of heat-inactivated Mycobacterium bovis and challenge with a field strain

	Sanidad y Biotecnología (SaBio). Instituto de Investigación en Recursos Cinegéticos (IREC). Consejo Superior de Investigaciones Científicas (CSIC). Universidad de Castilla La Mancha (UCLM). Gobierno de Castilla-La Mancha (JCCM).
	Indian Council of Agricultural Research (ICAR).
	Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Gobierno Vasco.
	Medianilla Red Deer Genetics.
	Servicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).