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Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs

The Veterinary Record publish this investigation article

March 1st, 1996

Samples from four dogs and four cats suspected of having tuberculosis were processed for histopathology, bacterial culture and polymerase chain reaction (PCR). A simple, rapid method for the extraction of DNA from tissue samples was used in two PCR assays designed to confirm the diagnosis of tuberculosis. The PCR assays detected all the culture-positive samples from these animals and no false positive results were obtained. The PCR technique was successful for the direct detection of organisms from the Mycobacterium tuberculosis complex and reduced the time needed for a diagnosis to two days




Aranaz A., Liebana E., Pickering X., Novoa C., Mateos A. and Dominguez L..




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Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs

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Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs



Participants:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







The Veterinary Record
FACTOR YEAR Q
1.017 2005

NLMID: 31164

PMID: 8711884

ISSN: 0042-4900



TITLE: Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs


JOURNAL: Vet Rec


NUMERACIÓN: 138(12):276-80


AÑO: 1996


PUBLISHER: British Veterinary Association


AUTHORS: Aranaz A., Liebana E., Pickering X., Novoa C., Mateos A. and Dominguez L..


Lucas Domínguez Rodríguez

DOI: https://doi.org/10.1136/vr.138.12.276


CITE THIS PUBLICATION:

Aranaz A., Liebana E., Pickering X., Novoa C., Mateos A. and Dominguez L. Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs. The Veterinary Record. 138(12):276-80. 1996. (A). ISSN: 0042-4900. DOI: 10.1136/vr.138.12.276


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