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Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices

Artículo de investigación publicado en Veterinary Microbiology

1 de mayo de 2019

Trueperella pyogenes is an opportunistic pathogen associated with a variety of diseases and responsible for important economic losses for pig production. Minimal Inhibitory Concentration (MIC) and Pulsed Field Gel Electrophoresis (PFGE) typing analysis were used to determine the MIC distribution and to genetically characterize a total of 180 T. pyogenes isolates obtained from slaughtered pigs reared under intensive (TpIN, n = 89) and extensive (TpEX, n = 91) farming practices. Low MIC90 values for penicillin and amoxicillin (0.008 and 0.06 μg/ml, respectively), ceftiofur, gentamicin and enrofloxacin (1 μg/ml, respectively) were obtained, so they could be of choice for the empiric treatment of T. pyogenes infections. Except for the penicillin, amoxicillin and ceftiofur, a statistically significant difference was observed in the MIC distribution of all antimicrobials analysed between TpIN and TpEX isolates. Also, MIC90 values were higher in TpIN than in TpEX isolates for neomycin and streptomycin (32 μg/ml vs 8 μg/ml), sulfamethoxazole/trimethoprim (30.4/1.6 μg/ml vs 1.90/0.10 μg/ml) and tylosin (≥1024 μg/ml vs 1 μg/ml). A relatively lower genetic diversity was detected in TpIN in comparison with TpEX isolates (GD 0.42 and GD 0.47, respectively). All isolates were distributed in three clusters (A, B, C). TpIN isolates were statistically associated with cluster A (P = 0.0002; OR 3.21; CI95 1.74-5.93), whereas the TpEX were distributed throughout the dendrogram, showing more genetic diversity. These data suggest that the antimicrobial susceptibility and genetic variability of the T. pyogenes isolates could be influenced by the management systems




Galan-Relaño A., Gomez-Gascon L., Luque I., Barrero-Dominguez B., Casamayor A., Cardoso-Toset F., Vela AI., Fernandez-Garayzabal JF. y Tarradas C.




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Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices

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Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices


Participantes:

Universidad de CórdobaDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad de Córdoba (UCO).

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Centro de Investigación y Calidad AgroalimentariaCentro de Investigación y Calidad Agroalimentaria (CICAP).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).







Veterinary Microbiology
FACTOR YEAR Q
2.791 2018

NLMID: 7705469

PMID: 31030851

ISSN: 0378-1135



TÍTULO: Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices


REVISTA: Vet. Microbiol.


NUMERACIÓN: 232:89-95


AÑO: 2019


EDITORIAL: Elsevier Science Publishers


AUTORES: Galan-Relaño A., Gomez-Gascon L., Luque I., Barrero-Dominguez B., Casamayor A., Cardoso-Toset F., Vela AI., Fernandez-Garayzabal JF. and Tarradas C.


PARTICIPANTES VISAVET


5th
Almudena Casamayor Sanz
7th
Ana Isabel Vela Alonso
8th
José Francisco Fernández-Garayzabal Fernández

DOI: https://doi.org/10.1016/j.vetmic.2019.04.011


CITA ESTA PUBLICACIÓN:

Galan-Relaño A., Gomez-Gascon L., Luque I., Barrero-Dominguez B., Casamayor A., Cardoso-Toset F., Vela AI., Fernandez-Garayzabal JF. y Tarradas C. Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices. Veterinary Microbiology. 232:89-95. 2019. (A). ISSN: 0378-1135. DOI: 10.1016/j.vetmic.2019.04.011


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