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ArmA methyltransferase in monophasic Salmonella enterica isolated from food

Investigación publicada en Antimicrobial Agents and Chemotherapy

1 de noviembre de 2011

The 16S rRNA methyltransferase ArmA is a worldwide emerging determinant that confers high-level resistance to most clinically relevant aminoglycosides. We report the identification and characterization of a multidrug resistant Salmonella enterica subspecies I. 4,12:i:- isolate recovered from chicken meat sampled in a supermarket on February 2009 in La Reunion, a French island in the Indian Ocean. Susceptibility testing showed an unusually high-level resistance to gentamicin as well as to ampicillin, third generation cephalosporins and amoxicillin/clavulanate. Molecular analysis of the 16S rRNA methyltransferases revealed presence of the armA gene together with blaTEM-1, blaCMY-2 and blaCTX-M-3. All these genes could be transferred en bloc through conjugation into E. coli at a frequency of 1 x 10-5 CFU/donor. Replicon typing and S1-PFGE revealed that the armA gene was borne on a broad-host-range IncP plasmid, pB1010, of approximately 150 kb in size. To elucidate how armA had integrated in pB1010, a PCR mapping strategy was developed for Tn1548, the genetic platform for armA. The gene was embedded in a Tn1548-like structure, albeit with a deletion of the macrolide resistance genes and an IS26 inserted within the mel gene. To our knowledge, this is the first report of the ArmA methyltransferase in the East of Africa and in food, showing a novel route of transmission for this resistance determinant. Further surveillance in food-borne bacteria will be crucial to determine the role of food in the spread of 16S rRNA methyltransferase genes worldwide




Granier SA., Hidalgo L., San Millan A., Escudero JA., Gutierrez B., Brisabois A. y Gonzalez-Zorn B..




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ArmA methyltransferase in monophasic Salmonella enterica isolated from food

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ArmA methyltransferase in monophasic Salmonella enterica isolated from food


Participantes:

Universidad ComplutenseServicio de Zoonosis de Transmisión Alimentaria y Resistencia a Antimicrobianos (ZTA). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Agence Nationale de Sécurité Sanitaire de l´alimentation, de l´environnement et du travailLaboratoire de Sécurité des Aliments. Agence Nationale de Sécurité Sanitaire de l´alimentation, de l´environnement et du travail (ANSES).







Antimicrobial Agents and Chemotherapy
FACTOR YEAR Q
4.841 2011

NLMID: 315061

PMID: 21859937

ISSN: 0066-4804



TÍTULO: ArmA methyltransferase in monophasic Salmonella enterica isolated from food


REVISTA: Antimicrob. Agents Chemother.


NUMERACIÓN: 55(11):5262-6


AÑO: 2011


EDITORIAL: American Society for Microbiology


AUTORES: Granier SA., Hidalgo L., San Millan A., Escudero JA., Gutierrez B., Brisabois A. and Gonzalez-Zorn B..


PARTICIPANTES VISAVET


4th
José Antonio Escudero García-Calderón
Last
Bruno González Zorn

DOI: https://doi.org/10.1128/AAC.00308-11


CITA ESTA PUBLICACIÓN:

Granier SA., Hidalgo L., San Millan A., Escudero JA., Gutierrez B., Brisabois A. y Gonzalez-Zorn B. ArmA methyltransferase in monophasic Salmonella enterica isolated from food. Antimicrobial Agents and Chemotherapy. 55(11):5262-6. 2011. (A). ISSN: 0066-4804. DOI: 10.1128/AAC.00308-11


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