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Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification

Capítulo en el libro Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases editado por Frontiers

1 de mayo de 2017

Perez-Sancho M., Vela AI., Garcia-Seco T., Gottschalk M., Dominguez L. y Fernandez-Garayzabal JF..


The accuracy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identifying Streptococcus suis isolates obtained from pigs, wild animals, and humans was evaluated using a PCR-based identification assay as the gold standard. In addition, MALDI-TOF MS was compared with the commercial multi-tests Rapid ID 32 STREP system. From the 129 S. suis isolates included in the study and identified by the molecular method, only 31 isolates (24.03%) had score values ≥2.300 and 79 isolates (61.24%) gave score values between 2.299 and 2.000. After updating the currently available S. suis MALDI Biotyper database with the spectra of three additional clinical isolates of serotypes 2, 7, and 9, most isolates had statistically significant higher score values (mean score: 2.65) than those obtained using the original database (mean score: 2.182). Considering the results of the present study, we suggest using a less restrictive threshold score of ≥2.000 for reliable species identification of S. suis. According to this cut-off value, a total of 125 S. suis isolates (96.9%) were correctly identified using the updated database. These data indicate an excellent performance of MALDI-TOF MS for the identification of S. suis





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Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification



Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

CEI Campus MoncloaCEI Campus Moncloa.

Faculté de Médicine Vétérinaire. Université de Montréal (UM).







Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification

CAPÍTULO: Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification


TIPO: Capítulo


AUTORES: Perez-Sancho M., Vela AI., Garcia-Seco T., Gottschalk M., Dominguez L. y Fernandez-Garayzabal JF..


PARTICIPANTES VISAVET


First
Marta Pérez Sancho
2nd
Ana Isabel Vela Alonso
3rd
Teresa García-Seco Romero
5th
Lucas Domínguez Rodríguez
Last
José Francisco Fernández-Garayzabal Fernández

LIBRO: Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases


EDICIÓN: 1


IDIOMA: Inglés


ISSN: 9782889451838


NUMERACIÓN: 113-118


FECHA: 1 de mayo de 2017


EDITORIAL: Frontiers


DOI: https://doi.org/10.3389/978-2-88945-183-8


CITA ESTA PUBLICACIÓN:

Perez-Sancho M., Vela AI., Garcia-Seco T., Gottschalk M., Dominguez L. y Fernandez-Garayzabal JF. Assessment of MALDI-TOF MS as alternative tool for Streptococcus suis identification. 113-118. Applications of STEM (Science, Technology, Engineering and Mathematics) tools in microbiology of infectious diseases. Ed. 1. Frontiers. ISBN: 9782889451838. 2017. (Capítulo)


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