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Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo

Artículo de investigación publicado en The Journal of Veterinary Medical Science

28 de diciembre de 2012

A swine-specific CpG motif enriched plasmid (pUC18-CpG) was constructed in this study. Its immunostimulant property was tested in vitro via lymphocyte transformation assay using swine peripheral blood mononuclear cells (PBMCs). The recombinant plasmid showed high Stimulation Index (SI) compared to the positive control (LPS). In a following animal vaccination-challenge experiment, pUC18-CpG was co-administered with a commercial swine FMD killed vaccine. Animals in the pUC18-CpG adjuvanted groups showed much higher antibody titers during the vaccination period




Guo X., Jia H., Yuan W., Zhang Q., Hou S., Sun Y., Zhu G., Zhu H. y Sanchez-Vizcaino JM..




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Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo

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Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo


Participantes:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Institute of Animal Science (IAS). Chinese Academy of Agricultural Sciences (CAAS).

Yangzhou UniversityCollege of Veterinary Medicine. Yangzhou University (YZU).

China Animal Husbandry Group Research Institute (CAHG).

Xinjiang Tiankang Animal Science Bio-Technology.







FACTOR YEAR Q
0.876 2012

NLMID: 9105360

PMID: 22814086

ISSN: 0916-7250



TÍTULO: Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo


REVISTA: J. Vet. Med. Sci.


NUMERACIÓN: 74(12):1647-50


AÑO: 2012


EDITORIAL: Japanese Society Of Veterinary Science


AUTORES: Guo X., Jia H., Yuan W., Zhang Q., Hou S., Sun Y., Zhu G., Zhu H. and Sanchez-Vizcaino JM..


PARTICIPANTES VISAVET


José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/10.1292/jvms.11-0543


CITA ESTA PUBLICACIÓN:

Guo X., Jia H., Yuan W., Zhang Q., Hou S., Sun Y., Zhu G., Zhu H. y Sanchez-Vizcaino JM. Construction of swine-specific CpG motif plasmid and the study of itsimmunostimulatory effects both in vitro and in vivo. The Journal of Veterinary Medical Science . 74(12):1647-50. 2012. (A). ISSN: 0916-7250. DOI: 10.1292/jvms.11-0543


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