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CRISPR elements within Campylobacter

Comunicación presentada en WVA/WMA Global Conference on One Health

21 de mayo de 2015

de Cardenas I., Fernandez-Garayzabal JF., de la Cruz ML., Dominguez L. y Gomez S.

Campylobacteriosis remains to be the most commonly reported food-borne zoonosis at the EU level (EFSA, 2014). Similarly to many bacteria and most Archaea, Campylobacter harbors an adaptative immune system, named as CRISPR-cas system, made of a succession of direct repeats interspersed by unique “spacers” sequences. CRISPR (Clustered regularly interspaced palindromic repeats)-cas system provide the protection of the cell against viral infection, conjugative plasmids and other foreign genetic elements through spacer acquisition. As spacers are incorporated in sequential order, CRISPR loci reflect the history of viral infection of the host, so they provide an ecological and epidemiological record (Marraffini; Sorek et al 2013).
We herein present a straightforward method consisting of a multiplex PCR and sequencing of the PCR products. We have designed the primers directed against all known Campylobacter CRISPR, both “confirmed” and “questionable”; these later had been disregarded up to date at genotyping. We were aware that, not only the combination “confirmed” + “questionable” is an excellent tool at Campylobacter genotyping, comparable or even better than other traditional typing methods such as flaA SVR, but also it allows traceability of the different strains. We also discuss the strong relationship among “confirmed” and “questionable” CRISPR





Participantes:

Universidad ComplutenseServicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).


Enlace a WVA/WMA Global Conference on One Health





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WVA/WMA Global Conference on One Health


21-22 mayo de 2015
Madrid
España

TÍTULO: CRISPR elements within Campylobacter


TIPO: Comunicación en póster


AUTORES: de Cardenas I., Fernandez-Garayzabal JF., de la Cruz ML., Dominguez L. y Gomez S.


PARTICIPANTES VISAVET


2nd
José Francisco Fernández-Garayzabal Fernández
4th
Lucas Domínguez Rodríguez

FECHA: 21 de mayo de 2015



CITA ESTA COMUNICACIÓN:

de Cardenas I., Fernandez-Garayzabal JF., de la Cruz ML., Dominguez L. y Gomez S. CRISPR elements within Campylobacter. WVA/WMA Global Conference on One Health, World Veterinary Association, World Medical Association, Madrid, España, 21 de mayo de 2015. (Comunicación en póster)


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