Detection of Leishmania infantum in samples of wild Leporidae by Direct Fluorescent Antibody (DFA) assay
Comunicación presentada en Med-Vet-Net Association 5th International Scientific Conference (OneHealth: Zoonoses - Emerging Threats)
27 de junio de 2017
Ortega-García MV., Moreno I., Garcia N., Dominguez M. y Salguero FJ.
Leishmania infantum is a protozoan parasite and one of the causative agent of leishmaniosis, a zoonosis and a neglected tropical disease (NTD). Canines are the main domestic animal reservoir, but recently, Leporidae have been described as competent reservoirs, being the source of infection during an important outbreak in Madrid (Spain) that has involved 697 human cases from 2009 to 2016, and it is still active. The detection of leishmaniosis in wild animals becomes a great challenge during surveillance programs in One Health initiatives. Therefore, the study of microscopic lesions and the detection of L. infantum by immunofluorescence staining in different tissues could add further insight onto the disease in wild Leporidae and would permit the precise localization of L. infantum in tissues for an eventual and more accurate DNA extraction process.
Campus La Marañosa (ITM). Instituto Nacional de Técnica Aeroespacial (INTA). Ministerio de Defensa. | |
Centro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII). | |
Servicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM). | |
School of Veterinary Medicine. Faculty of Health and Medical Sciences. University of Surrey. | |
Enlace a Med-Vet-Net Association 5th International Scientific Conference (OneHealth: Zoonoses - Emerging Threats)