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Detection of Leishmania-specific DNA and surface antigens using a combination of functionalized magnetic beads and cadmium selenite quantum dots

Artículo de investigación publicado en Journal of microbiological methods

1 de abril de 2016

Leishmaniosis is a zoonotic disease that affects millions of people especially in resource-poor settings. The development of reliable diagnostic assays that do not require dedicated equipment or highly trained personnel would improve early diagnosis and effective control. For this purpose, a combination of magnetic bead and cadmium selenite quantum dot probes was applied for the detection of Leishmania-specific surface antigens (proteins) and DNA. Both analytes are isolated from the solution using magnetic bead capture probes whereas the presence of the targeted molecules is demonstrated by quantum dot detection probes. The sensitivity and specificity of this method reached 100% based on an assessment performed on 55 cultured isolates of various microbial pathogens. The low limit of detection was 3125ng/μl and 103cells/ml for Leishmania DNA and protein, respectively. The method shows considerable potential for clinical application in human and veterinary medicine, especially in resource-poor settings




Andreadou M., Liandris E., Gazouli M., Mataraqka A., Tachtsidis I., Goutas N., Vlachodimitropoulos D. y Ikonomopoulos J.




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Detection of Leishmania-specific DNA and surface antigens using a combination of functionalized magnetic beads and cadmium selenite quantum dots

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Detection of Leishmania-specific DNA and surface antigens using a combination of functionalized magnetic beads and cadmium selenite quantum dots


Participantes:

Agricultural University of AthensLaboratory of Anatomy and Physiology of Farm Animals. Faculty of Animal Health and Aquaculture. Agricultural University of Athens (AUA).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

National and Kapodistrian University of AthensDepartment of Forensic Medicine and Toxicology. Laboratory of Biology. Faculty of Medicine. National and Kapodistrian University of Athens (UOA).

University College LondonDepartment of Medical Physics and Bioengineering. Biomedical Optics Research Laboratory. University College London (UCL).







Journal of microbiological methods
FACTOR YEAR Q
1.790 2016

NLMID: 8306883

PMID: 26658854

ISSN: 0167-7012



TÍTULO: Detection of Leishmania-specific DNA and surface antigens using a combination of functionalized magnetic beads and cadmium selenite quantum dots


REVISTA: J Microbiol Methods


NUMERACIÓN: 123:62-67


AÑO: 2016


EDITORIAL: Amsterdam, The Netherlands : Elsevier Biomedical, c1983-


AUTORES: Andreadou M., Liandris E., Gazouli M., Mataraqka A., Tachtsidis I., Goutas N., Vlachodimitropoulos D. and Ikonomopoulos J.


DOI: https://doi.org/10.1016/j.mimet.2015.11.019


CITA ESTA PUBLICACIÓN:

Andreadou M., Liandris E., Gazouli M., Mataraqka A., Tachtsidis I., Goutas N., Vlachodimitropoulos D. y Ikonomopoulos J. Detection of Leishmania-specific DNA and surface antigens using a combination of functionalized magnetic beads and cadmium selenite quantum dots. Journal of microbiological methods. 123:62-67. 2016. (A). ISSN: 0167-7012. DOI: 10.1016/j.mimet.2015.11.019


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