Applied and Environmental Microbiology publica este artículo de investigación

1 de enero de 1999

A PCR-based method was developed for the specific detection of Yersinia ruckeri in tissues of inoculated trout and naturally infected trout. No amplification products were obtained with other yersiniae, bacterial fish pathogens, or phylogenetically related bacteria (n = 34). The sensitivity of PCR detection was 60 to 65 bacterial cells per PCR tube, which was decreased to 10 to 20 cells by hybridization with a nonradioactive probe. The PCR assay proved to be as reliable as and faster than the conventional culture method for the detection of Y. ruckeri in infected trout tissues

Gibello A., Blanco MM., Moreno MA., Cutuli MT., Domenech A., Dominguez L. y Fernandez-Garayzabal JF..

Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout

Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout

	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).