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Evaluation of P22 antigenic complex for the immuno-diagnosis of tuberculosis in BCG vaccinated and unvaccinated goats

Artículo de investigación publicado en Frontiers in veterinary science

3 de julio de 2020

Current eradication strategies of tuberculosis (TB) in goats resemble bovine TB eradication programs and mainly relay on single and comparative intradermal tuberculin tests (SITT and CITT). TB vaccination has been proposed as a cost-effective option in high prevalent herds or countries were economic compensation for slaughter positive animals is not affordable. However, TB vaccination compromises the efficiency of TB standard diagnostic tests. In this study, the performance of a new diagnostic platform, based on the P22 antigenic complex, was assessed for skin test (ST), Interferon gamma release assay (IGRA) and serology under different TB scenarios. The sensitivity (Se) of diagnostic tests was assessed in TB infected goats from the same farm (herd A, N=77) and the specificity (Sp) was assessed in two TB negative farms, one TB unvaccinated (herd B, N=77) and another vaccinated with BCG (herd C, N= 68). The single (s) P22-IGRA showed the highest Se among IGRA tests (91%) and the comparative (c) P22-ST showed the highest Sp (100% in Herd B and 98% in Herd C). Combined interpretation of techniques enabled the best diagnostic performances. Combining the CITT + sP22-IGRA improved Se (97%) compared to CITT + tuberculin-based-IGRA (95%), with a reduction of Sp (95% and 100%, respectively). In addition, combination of P22-ELISA + cP22-ST or CITT elicited similar performance in the non-vaccination context (Se: 94% and 95%; Sp: 95% and 95%, respectively) but Sp was significantly higher in the combination with cP22-ST compared to CITT in vaccination context (95% and 79%, respectively). The combination of serological tests based on P22 and MPB83 showed higher complementarity and improved 13% the Se of P22-ELISA alone. These findings suggest that either cell-mediated or antibodies based diagnostic techniques, using the P22 antigen complex, can contribute to improve the immunodiagnostic of TB in goats under different TB control strategies




Arrieta-Villegas CC., Infantes-Lorenzo JA., Bezos J., Grasa M., Vidal E., Mercader I., Singh M., Domingo M., de Juan L. y Perez del Val B.




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Evaluation of P22 antigenic complex for the immuno-diagnosis of tuberculosis in BCG vaccinated and unvaccinated goats

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Evaluation of P22 antigenic complex for the immuno-diagnosis of tuberculosis in BCG vaccinated and unvaccinated goats


Participantes:

Generalitat de CatalunyaInstituto de Investigación y Tecnología Agroalimentarias (IRTA). Generalitat de Catalunya.

Universitat Autònoma de BarcelonaCentre de Recerca en Sanitat Animal (CReSA). Universitat Autònoma de Barcelona (UAB).

Instituto de Salud Carlos IIICentro Nacional de Microbiología (CNM). Instituto de Salud Carlos III (ISCIII).

Universidad ComplutenseServicio de Micobacterias (MYC). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Generalitat de CatalunyaDepartamento de Agricultura, Ganadería, Pesca, Alimentación y Medio Natural. Generalitat de Catalunya.

Universitat Autònoma de BarcelonaDepartamento de Sanidad y de Anatomía Animal. Facultad de Veterinaria. Universitat Autònoma de Barcelona (UAB).







Frontiers in veterinary science
FACTOR YEAR Q
2.245 2019

NLMID: 101666658

PMID: 32714950

ISSN: 2297-1769



TÍTULO: Evaluation of P22 antigenic complex for the immuno-diagnosis of tuberculosis in BCG vaccinated and unvaccinated goats


REVISTA: Front Vet Sci


NUMERACIÓN: 7:374


AÑO: 2020


EDITORIAL: Lausanne : Frontiers Media S.A


AUTORES: Arrieta-Villegas CC., Infantes-Lorenzo JA., Bezos J., Grasa M., Vidal E., Mercader I., Singh M., Domingo M., de Juan L. and Perez del Val B.


PARTICIPANTES VISAVET


3rd
Javier Bezos Garrido
9th
Lucía de Juan Ferré

DOI: https://doi.org/10.3389/fvets.2020.00374


CITA ESTA PUBLICACIÓN:

Arrieta-Villegas CC., Infantes-Lorenzo JA., Bezos J., Grasa M., Vidal E., Mercader I., Singh M., Domingo M., de Juan L. y Perez del Val B. Evaluation of P22 antigenic complex for the immuno-diagnosis of tuberculosis in BCG vaccinated and unvaccinated goats. Frontiers in veterinary science. 7:374. 2020. (A). ISSN: 2297-1769. DOI: 10.3389/fvets.2020.00374


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