Applied and Environmental Microbiology publica este artículo de investigación

1 de julio de 2013

Lactococcus garvieae 21881, isolated from a human clinical case, produces a novel class IId bacteriocin, garvicin A (GarA), which is specifically active against other L. garvieae strains, including fish and bovine pathogenic isolates. Purification from active supernatants, sequence analyses, and plasmid-curing experiments identified pGL5, one of the five plasmids found in L. garvieae 21881 (Aguado-Urda M, Gibello A, Blanco MM, López-Campos GH, Cutuli MT, Fernández-Garayzábal JF. 2012. PLoS ONE 7 (6):e40119. doi:10.1371/journal.pone.0040119), as the coding plasmid for the structural gene of GarA (lgnA), its putative immunity protein (lgnI), and the ABC-transporter and its accessory protein (lgnC and lgnD). Interestingly, pGL5-cured strains were still resistant to GarA. Other putative bacteriocins encoded by the remaining plasmids were not detected during purification pointing to GarA as the main inhibitor secreted by L. garvieae 21881. Mode of action studies revealed a potent bactericidal activity of GarA. Moreover, transmission microscopy showed that GarA seems to act by inhibiting septum formation in L. garvieae cells. The potent and species-specific inhibition by GarA anticipates promising applications for the prevention or treatment of infections caused by pathogenic strains of L. garvieae in both veterinary and clinical settings

Maldonado-Barragan A., Cardenas N., Martinez-Fernandez B., Ruiz-Barba JL., Fernandez-Garayzabal JF., Rodriguez JM. y Gibello A.

Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains

Garvicin A, a novel class IId bacteriocin from Lactococcus garvieae that inhibits septum formation in L. garvieae strains

	Instituto de la Grasa. Consejo Superior de Investigaciones Científicas (CSIC).
	Departamento de Nutrición, Bromatología y Tecnología de los Alimentos. Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).
	Instituto de Productos Lácteos de Asturias (IPLA). Consejo Superior de Investigaciones Científicas (CSIC).
	Servicio de Identificación y Caracterización Microbiana (ICM). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).
	Departamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).