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Immunohistochemical Detection of Bluetongue Virus in Fixed Tissue

Journal of Comparative Pathology publica este artículo de investigación

1 de julio de 2010

The VP7 structural protein is the most abundant of the major core proteins and is highly conserved in all serotypes of bluetongue virus (BTV). The aim of this study was to develop immunohistochemical techniques for the detection of BTV VP7 in Bouin`s- and formalin-fixed and paraffin wax-embedded tissues from small ruminants (sheep and goats) naturally infected with BTV. Tissue samples were taken from animals in which BTV infection had been confirmed by reverse transcriptase polymerase chain reaction. Optimal results were obtained by incubation of monoclonal antibody 2E9 on samples fixed with Bouin`s solution or neutral buffered formalin. Optimum antigen retrieval for Bouin`s-fixed samples was by microwave heating (6min) of tissue samples in citrate buffer (pH 6.0, 0.01M), while for formalin-fixed samples a 30min heating period in pH 9.0 buffer was required. In both species, BTV was mainly detected in the spleen, lymph nodes and lungs; specifically within the arteriolar and capillary endothelial cells, together with macrophages and lymphocytes. The immunohistochemical method described will be a useful tool for future research. Copyright © 2010 Elsevier Ltd. All rights reserved




Sanchez-Cordon PJ., Rodriguez B., Risalde MA., Molina V., Pedrera M., Sanchez-Vizcaino JM. y Gomez-Villamandos JC.




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Immunohistochemical Detection of Bluetongue Virus in Fixed Tissue

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Immunohistochemical Detection of Bluetongue Virus in Fixed Tissue


Participantes:

Universidad ComplutenseServicio de Inmunología Viral y Medicina Preventiva (SUAT). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad de CórdobaDepartamento de Anatomía y Anatomía Patológica Comparadas. Facultad de Veterinaria. Universidad de Córdoba (UCO).







Journal of Comparative Pathology
FACTOR YEAR Q
1.529 2010

NLMID: 102444

PMID: 20156627

ISSN: 0021-9975



TÍTULO: Immunohistochemical Detection of Bluetongue Virus in Fixed Tissue


REVISTA: J. Comp. Pathol.


NUMERACIÓN: 143(1):20-28


AÑO: 2010


EDITORIAL: Academic Press


AUTORES: Sanchez-Cordon PJ., Rodriguez B., Risalde MA., Molina V., Pedrera M., Sanchez-Vizcaino JM. and Gomez-Villamandos JC.


PARTICIPANTES VISAVET


José Manuel Sánchez-Vizcaíno Rodríguez

DOI: https://doi.org/10.1016/j.jcpa.2009.12.017


CITA ESTA PUBLICACIÓN:

Sanchez-Cordon PJ., Rodriguez B., Risalde MA., Molina V., Pedrera M., Sanchez-Vizcaino JM. y Gomez-Villamandos JC. Immunohistochemical Detection of Bluetongue Virus in Fixed Tissue. Journal of Comparative Pathology. 143(1):20-28. 2010. (A). ISSN: 0021-9975. DOI: 10.1016/j.jcpa.2009.12.017


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