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Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization

Investigación publicada en FEMS Microbiology Immunology

1 de julio de 1992

A group of 14 monoclonal antibodies (mAbs) to staphylococcal enterotoxin B (SEB) were obtained by fusion of Sp2/O myeloma cells with spleen cells from female BALB/c mice immunized with commercial SEB. The antibodies belonged to IgG1 and IgG2b subclasses. We evaluated the anti-SEB titres, competition assays and sensitivity of detection by indirect ELISA. Reactivity and cross-reactivity were also studied by indirect ELISA and confirmed by immunoblotting. All the mAbs reacted with SEB and with a second band which had a different electrophoretic mobility and probably represents an aggregate of SEB or SEB bound to membranes. Three mAbs reacted only with SEB and the rest showed cross-reactions with SEC1. No reactions were observed against any other serovar (SEA, SED and SEE) or other proteins




Goyache J., Orden JA., Blanco JL., Hernandez FJ., Domenech A., Suarez G. y Gomez-Lucia E.




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Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization

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Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization


Participantes:

Universidad ComplutenseDepartamento de Sanidad Animal. Facultad de Veterinaria. Universidad Complutense (UCM).

Universidad ComplutenseServicio de Zoonosis Emergentes, de Baja Prevalencia y Agresivos Biológicos (NED). Centro de Vigilancia Sanitaria Veterinaria (VISAVET). Universidad Complutense (UCM).







FACTOR YEAR Q
1.006 1992

NLMID: 8901230

PMID: 1515153

ISSN: 0920-8534



TÍTULO: Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization


REVISTA: FEMS Microbiol Immunol


NUMERACIÓN: 4(5):247-54


AÑO: 1992


EDITORIAL: Elsevier Science Publishers


AUTORES: Goyache J., Orden JA., Blanco JL., Hernandez FJ., Domenech A., Suarez G. and Gomez-Lucia E.


PARTICIPANTES VISAVET


First
Joaquín Goyache Goñi
6th
Guillermo Suárez Fernández

DOI: https://doi.org/10.1111/j.1574-6968.1992.tb05002.x


CITA ESTA PUBLICACIÓN:

Goyache J., Orden JA., Blanco JL., Hernandez FJ., Domenech A., Suarez G. y Gomez-Lucia E. Murine monoclonal antibodies against staphylococcal enterotoxin B: production and characterization. FEMS Microbiology Immunology. 4(5):247-54. 1992. (A). ISSN: 0920-8534. DOI: 10.1111/j.1574-6968.1992.tb05002.x


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